CE3C-Center for Ecology, Evolution and Environmental Changes, Faculty of Sciences, University of Lisbon, Campo Grande, Portugal.
Faculty of Sciences, Department of Plant Biology, University of Lisbon, Campo Grande, Portugal.
Biotech Histochem. 2023 May;98(4):291-295. doi: 10.1080/10520295.2023.2182452. Epub 2023 Mar 7.
The cyanophycin content of the heterocystous nitrogen-fixing symbiotic cyanobacterium, , which inhabits an ovoid cavity in the dorsal leaf lobes of the fern, , is seldom analyzed. To study the cyanophycin content in vegetative cells and heterocysts of , we used three fluorochromes: aluminum trichloride, lead citrate and Wilson citroboric solution and Coomassie brilliant blue. Blue and yellow fluorescence were emitted from the polar nodes and cytoplasm cyanophycin granules of the heterocysts when stained with the three fluorochromes. The cyanophycin observed without staining or with Coomassie brilliant blue staining did not alter the results obtained using the fluorochromes. We found that aluminum trichloride, lead acetate and Wilson citroboric solution could be used to detect cyanophycin.
异形胞固氮共生蓝藻的藻青素含量很少被分析，该蓝藻栖息于蕨类植物背叶裂片的卵形腔中。为了研究的藻青素含量，我们使用了三种荧光染料：三氯化铝、柠檬酸铅和威尔逊柠檬酸硼溶液以及考马斯亮蓝。用这三种荧光染料染色后，异形胞的极节和细胞质藻青素颗粒发出蓝色和黄色荧光。未经染色或用考马斯亮蓝染色观察到的藻青素并不改变使用荧光染料获得的结果。我们发现三氯化铝、醋酸铅和威尔逊柠檬酸硼溶液可用于检测藻青素。
