Kardish Nitza, Ronen R, Bubrick P, Garty J
Department of Botany, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.
Institute for Nature Conservation Research, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.
New Phytol. 1987 Aug;106(4):697-706. doi: 10.1111/j.1469-8137.1987.tb00170.x.
The most efficient of five methods used for the extraction of ATP from lichens was found to be the use of Tris + EDTA after acetone treatment. Changes in the concentration of ATP in transplanted Ramalina duriaei (De Not.) Bagl. reflected the influence of air pollution on this lichen. The ATP concentration remained constant at a control station in a rural site, whilst at urban stations it decreased to 10% of the control value. Changes in the concentration of ATP were compared with chlorophyll degradation, as expressed by the ratio OD 435 nm/OD 415 nm. The values of this ratio also decreased in the urban sites, but less so than the ATP.
在用于从地衣中提取ATP的五种方法中，最有效的方法是在丙酮处理后使用Tris + EDTA。移植的硬枝树花衣（De Not.）Bagl.中ATP浓度的变化反映了空气污染对该地衣的影响。在农村地区的一个对照站点，ATP浓度保持恒定，而在城市站点，其浓度降至对照值的10%。将ATP浓度的变化与叶绿素降解进行比较，叶绿素降解用OD 435 nm/OD 415 nm比值表示。该比值在城市站点也有所下降，但降幅小于ATP。
