Foissner Ilse
Institut für Pflanzenphysiologie, Universität Salzburg, Hellbrunnerstrafße 34, A-5020 Salzburg, Austria.
New Phytol. 1992 Jul;121(3):447-455. doi: 10.1111/j.1469-8137.1992.tb02945.x.
The effect of dichlorobenzonitrile (DCB), an inhibitor of cellulose synthesis, on the formation of local cell wall appositions (plugs) was studied in internodal cells of the characeae Chara corallina Klein ex. Wild, em. R.D.W. and Nitella Flexilis (L.) Ag. DCB Significantly reduces size and number of fibrillar, membrane-free plugs induced by 6 × 10 M Cacl . Formation of these plugs leads to an increase in the cellulose content which is abolished in the presence of DCB. Plugs formed during treatment with DCB have a less regular structure, but membrane recycling appears not to be disturbed. Cells are able to recover from DCB treatment and normal plugs are then formed. DCB has only minor effects on size, number and structure of amorphous, membrane-containing plugs induced by chlortetracycline (CTC) although their callose content is significantly enhanced. The data confirm that fibrillar plugs consist mainly of crystalline cellulose and that this polysaccharide does not play any role in the formation of amorphous plugs.
研究了纤维素合成抑制剂二氯苯腈（DCB）对轮藻科珊瑚轮藻（Chara corallina Klein ex. Wild, em. R.D.W.）和柔轮藻（Nitella Flexilis (L.) Ag.）节间细胞中局部细胞壁附着物（栓塞）形成的影响。DCB显著减小了由6×10⁻⁶ M氯化钙诱导形成的纤维状、无膜栓塞的大小和数量。这些栓塞的形成会导致纤维素含量增加，而在DCB存在的情况下这种增加被消除。在DCB处理过程中形成的栓塞结构不太规则，但膜循环似乎未受干扰。细胞能够从DCB处理中恢复，随后形成正常的栓塞。DCB对金霉素（CTC）诱导形成的无定形、含膜栓塞的大小、数量和结构影响较小，尽管其胼胝质含量显著增加。数据证实纤维状栓塞主要由结晶纤维素组成，且这种多糖在无定形栓塞的形成中不起任何作用。
