Boudarga Khadija, Lapeyrie Fréderié, Dexheimer Jean
Laboratoire de Biologie des Ligneux, JE CNRS 034613, Université de Nancy I, BP 239, 54506 Vandoeuvre Cedex, France.
INRA, Centre de Recherches Forestières de Nancy, Champenousx, 54280 Seichamps, France.
New Phytol. 1990 Jan;114(1):73-76. doi: 10.1111/j.1469-8137.1990.tb00375.x.
A few species of plant, including Eucalyptus, bear simultaneously on their root system and even on the same root apex, more than one symbiotic microorganism. The development of techniques allowing mixed inoculations is an essential prerequisite for the study of interactions between different symbionts. A simple in vitro technique for rapid synthesis of dual vesicular-arbuscular endomycorrhiza/ectomycorrhiza on a single root apex of Eucalyptus seedlings is described. This technique was derived from the paper-sandwich technique for synthesis of ectomycorrhizas, and was modified to render it compatible with endomycorrhizal infection. Electron microscopy observations of dual endomycorrhiza/ectomycorrhiza showed both symbioses coexisting on the same apex, with no obvious sign of antagonism.
包括桉树在内的少数几种植物，其根系甚至在同一根尖上同时存在不止一种共生微生物。开发允许混合接种的技术是研究不同共生体之间相互作用的必要前提。本文描述了一种简单的体外技术，用于在桉树苗的单个根尖上快速合成双泡囊 - 丛枝内生菌根/外生菌根。该技术源自用于合成外生菌根的纸三明治技术，并经过改良以使其与内生菌根感染兼容。双内生菌根/外生菌根的电子显微镜观察表明，两种共生体在同一根尖上共存，没有明显的拮抗迹象。
