Haigh G R, Carver T L W, Gay A P, Farrar J F
School of Biological Sciences, University College of North Wales, Bangor, Gwynedd LL57 2UW, U.K.
AFRC Institute of Grassland and Environmental Research, Welsh Plant Breeding Station, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB, U.K.
New Phytol. 1991 Sep;119(1):129-136. doi: 10.1111/j.1469-8137.1991.tb01016.x.
Rates of photosynthesis and respiration were measured by two methods, oxygen electrode and infrared gas analysis (IRGA), in infected and control, seedling and adult leaves of oat genotypes exhibiting different levels of partial resistance. Measurements were carried out up to 9 d after inoculation, sporulation commencing on day 5. There was no decrease in the rate of photosynthesis, except in the second leaves of one genotype, and no decrease in any of the fifth leaves relative to controls. The two methods of measuring respiration gave different results, with no consistent differences being found. Measurements by oxygen electrode, up to 6 d after inoculation, revealed that disease treatment had a significant effect on respiration in only two cases; the susceptible first leaf of one genotype and the resistant fifth leaf of another. Measurements by IRGA, up to 9 d after inoculation, did not confirm these differences, but did reveal a significant increase in respiration in the diseased fifth leaf of another resistant genotype. SHAM inhibited respiration, indicating the presence of the alternative oxidase, but there was no significant difference in its activity between diseased and control plants. Thus changes in photosynthesis and respiration previously associated with powdery mildew infection are either delayed or compensated for in oats. Photosynthetic and respiratory responses do not appear to be involved in partial resistance of oats to powdery mildew.
采用氧电极法和红外气体分析法（IRGA）这两种方法，对表现出不同水平部分抗性的燕麦基因型的感染叶和对照叶、幼苗叶和成年叶的光合作用和呼吸作用速率进行了测定。在接种后长达9天进行测量，第5天开始产孢。除了一种基因型的第二片叶子外，光合作用速率没有下降，相对于对照，第五片叶子均未出现下降。两种测量呼吸作用的方法得出了不同的结果，未发现一致的差异。接种后6天内用氧电极测量发现，只有两种情况下病害处理对呼吸作用有显著影响；一种基因型的感病第一片叶子和另一种基因型的抗病第五片叶子。接种后9天内用IRGA测量未证实这些差异，但确实发现另一种抗病基因型的患病第五片叶子的呼吸作用显著增加。水杨基氧肟酸（SHAM）抑制呼吸作用，表明存在交替氧化酶，但患病植株和对照植株之间其活性没有显著差异。因此，先前与白粉病感染相关的光合作用和呼吸作用变化在燕麦中要么延迟要么得到补偿。光合作用和呼吸作用响应似乎与燕麦对白粉病的部分抗性无关。
