不同养分状况对挪威云杉[欧洲云杉（Picea abies (L.) Karst.）]幼苗碳代谢调节的影响。

Influence of different nutrient regimes on the regulation of carbon metabolism in Norway spruce [Picea abies (L.) Karst.] seedlings.

作者信息

Wingler Astrid, Einig Werner, Schaeffer Christoph, Wallenda Thomas, Hampp Rüdiger, Wallander Håkan, Nylund Jan-Erik

机构信息

Physiologische Okoiogie der Pflamsen, Botanisches Institute Universitat Tubingen, Auf der Morgenstelle 1, D-72076 Tubingen, Germany.

Department of Microbial Ecology, University of Lund, Helgonavagen 5, S-223 62 Lund, Sweden.

出版信息

New Phytol. 1994 Oct;128(2):323-330. doi: 10.1111/j.1469-8137.1994.tb04016.x.

DOI:10.1111/j.1469-8137.1994.tb04016.x

PMID:33874368

Abstract

Phosphoenolpyruvate carboxylase (PEPC) activity, fructose 2, 6-bisphosphate (F26BP), starch and soluble sugar contents were determined m needles and roots of Norway spruce seedlings grown in a semi-hydroponic cultivation system under different nutrient regimes, tn needles, a surplus of nitrogen caused an increase in specific PEPC activity (up to six times control activity) and F26BP content (up to three times control level) while starch content was reduced. Sucrose contents were not affected. Basically, the responses in root samples were similar. Here, PEPC was highest at an imbalance in nutrition (+ N/ -P) F26BP, with root contents being 3- to 11 -times higher than those in needles, significantly exceeded control values at + N/+ P. The results show that alteration of nitrogen supply leads to marked changes in allocation of carbon between pathways, which is also influenced by P-nutrition. Pool sizes of F26BP and activity of PEPC are indicators for these changes in leaf as well as in root tissues of Norway spruce.

摘要

在不同养分条件下，于半水培栽培系统中生长的挪威云杉幼苗的针叶和根系中，测定了磷酸烯醇式丙酮酸羧化酶（PEPC）活性、果糖-2,6-二磷酸（F26BP）、淀粉和可溶性糖含量。在针叶中，氮素过剩导致比活性PEPC增加（高达对照活性的6倍）和F26BP含量增加（高达对照水平的3倍），而淀粉含量降低。蔗糖含量未受影响。基本上，根系样本中的响应相似。在此，PEPC在营养失衡（+N/-P）时最高，F26BP的根系含量比针叶中的高3至11倍，在+N/+P时显著超过对照值。结果表明，氮供应的改变导致碳在不同途径间分配的显著变化，这也受磷营养的影响。F26BP的库大小和PEPC的活性是挪威云杉叶片和根系组织中这些变化的指标。