Bradbury S M, Peterson R L, Bowley S R
Department of Botany, University of Guelph, Guelph, Ontario, NIG 2W1, Canada.
Department of Crop Science, University of Guelph, Guelph, Ontario, NIG 2W1, Canada.
New Phytol. 1993 Aug;124(4):665-673. doi: 10.1111/j.1469-8137.1993.tb03856.x.
The vesicular-arbuscular (VA) mycorrhizal status of three alfalfa (Medicago sativa L.) nodulation genotypes (nod fix , nod fix and nod fix ) was investigated using pot cultures of three fungal species from two genera (Glomus monosporum Gerd. & Trappe, Glomus fasciculatum (Thaxter) Gerd. & Trappe emend. Walker & Koske and Gigaspora margarita Becker & Hall). Roots of the nod fix genotype developed complete VA mycorrhizas with each fungus. Roots of the nod fix and nod fix genotypes did not develop normal VA mycorrhizal symbioses. Roots of the nod fix genotype had significantly more appressoria than roots of the other genotypes. Aborted appressoria were unable to penetrate the cortical cells of roots of nod fix and nod fix genotypes. Measurement of appressorium length, width and approximate contact area revealed significant differences in the size of appressoria produced by each VA mycorrhizal fungus on each alfalfa nodulation genotype. Histological studies of nod fix and nod fix genotypes colonized by Glomus versiforme (Karsten) Berch showed the presence of electron-dense material in aborted appressoria on roots of the nod fix genotype but not in appressoria on roots of the nod fix genotype.
利用来自两个属的三种真菌（单孢球囊霉Gerd. & Trappe、聚丛球囊霉（Thaxter）Gerd. & Trappe emend. Walker & Koske和珠状巨孢囊霉Becker & Hall）的盆栽培养物，研究了三种苜蓿（紫花苜蓿L.）结瘤基因型（nod fix、nod fix和nod fix）的泡囊-丛枝（VA）菌根状态。nod fix基因型的根与每种真菌都形成了完整的VA菌根。nod fix和nod fix基因型的根没有形成正常的VA菌根共生体。nod fix基因型的根比其他基因型的根有更多的附着胞。 aborted附着胞无法穿透nod fix和nod fix基因型根的皮层细胞。对附着胞长度、宽度和近似接触面积的测量显示，每种VA菌根真菌在每种苜蓿结瘤基因型上产生的附着胞大小存在显著差异。对被多形球囊霉（Karsten）Berch定殖的nod fix和nod fix基因型进行的组织学研究表明，在nod fix基因型根上的aborted附着胞中存在电子致密物质，而在nod fix基因型根上的附着胞中则不存在。
