Lagrange Hubert, Jay-Allgmand Christian, Lapeyrie Frédéric
Equipe de Microbiologie Forestière, Centre de Recherches de Nancy, Institut National de la Recherche Agronomique, F-54280 Champenoux, France.
Station d'Amélioration des Arbres Forestiers, Centre de Recherches d'Orléans, Institut National de la Recherche Agronomique, F-45160 Olivet, France.
New Phytol. 2001 Feb;149(2):349-355. doi: 10.1046/j.1469-8137.2001.00027.x.
• Ectomycorrhizal hyphal growth is shown to be stimulated by a phenol compound isolated from Eucalyptus globulus ssp. bicostata root exudates, highlighting the importance of phenolics in host-fungal interaction. • HPLC analysis allowed separation and identification of phenolic compounds from Eucalyptus seedling tissues and root exudates. The activity of the flavonol, rutin, was tested on a range of mycorrhizal and saprophytic fungi. • Rutin stimulated Pisolithus hyphal growth by more than twofold, and the fungus responded significantly to concentrations as low as 1 pM; only a few strains responded. • Rutin from Eucalyptus globulus ssp. bicostata root exudates is a flavonoid signal for Pisolithus, and is the first such flavonoid signal identified. A rutin gradient could contribute to orientating hyphal elongation toward the root tip thereby favouring mycorrhizal infection, and might also influence the interaction between fungi in the rhizosphere.
• 从蓝桉双肋亚种根系分泌物中分离出的一种酚类化合物可刺激外生菌根菌丝生长，这突出了酚类物质在宿主 - 真菌相互作用中的重要性。
• 高效液相色谱分析能够分离并鉴定桉树苗组织和根系分泌物中的酚类化合物。对黄酮醇芦丁的活性在一系列菌根真菌和腐生真菌上进行了测试。
• 芦丁刺激硬皮马勃菌丝生长超过两倍，该真菌对低至1皮摩尔的浓度有显著反应；只有少数菌株有反应。
• 蓝桉双肋亚种根系分泌物中的芦丁是硬皮马勃的一种类黄酮信号，是首个被鉴定出的此类类黄酮信号。芦丁梯度可能有助于引导菌丝向根尖伸长，从而有利于菌根感染，也可能影响根际真菌之间的相互作用。
