Albrecht C, Burgess T, Dell B, Lapeyrie F
INRA, Centre de Recherches Forestières de Nancy, 54280 Champenoux- France.
Murdoch University, School of Biological Sciences, Perth, Australia 6150.
New Phytol. 1994 Jun;127(2):217-222. doi: 10.1111/j.1469-8137.1994.tb04273.x.
Peroxidase and chitinase activities were measured in roots of Eucalyptus globulus spp bicostata Kirkp. during colonization by Pisolithus sp. isolated from under Eucalyptus. Ten fungal isolates, ranging from poor to good root colonizers, were selected to represent a range of ectomycorrhizal aggressivity. The induction of chitinases and peroxidases was strongly related to the aggressiveness of the fungal strain. Only good colonizers, that is strains which rapidly form differentiated ectomycorrhizas, induced a strong response in the plant. Therefore, it can be concluded that these enzymes are not responsible for poor root colonization by the less aggressive strains. The chitinase response of Eucalyptus roots to contact with fungal extracts differed only slightly between weakly and strongly aggressive strains. This suggests that a major component of differential induction observed in vivo is the consequence of root colonization, tissue penetration and the ability to deliver elicitors to the plain print to and during rant colonization.
在被从桉树下分离出的 Pisolithus sp. 定殖期间，对蓝桉双肋变种柯克普的根中的过氧化物酶和几丁质酶活性进行了测定。从根定殖能力差到强的范围内选择了 10 种真菌分离物，以代表一系列外生菌根的侵染性。几丁质酶和过氧化物酶的诱导与真菌菌株的侵染性密切相关。只有定殖能力强的菌株，即能迅速形成分化良好的外生菌根的菌株，才会在植物中诱导出强烈的反应。因此，可以得出结论，这些酶并非导致侵染性较弱的菌株对根定殖不良的原因。蓝桉根对与真菌提取物接触的几丁质酶反应，在侵染性弱和强的菌株之间仅略有差异。这表明，在体内观察到的差异诱导的一个主要成分是根定殖、组织穿透以及在根定殖期间将激发子传递到植物体内的能力的结果。
