Department of Obstetrics and Gynecology, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, China.
Immunopharmacol Immunotoxicol. 2021 Jun;43(3):334-342. doi: 10.1080/08923973.2021.1910834. Epub 2021 Apr 20.
Preeclampsia (PE) is an idiopathic hypertensive disorder of pregnancy, which is related to abnormal placental villi development. Our previous study has found that lncRNA NEAT1 promotes apoptosis of trophoblasts, but the role of NEAT1 in proliferation, migration, and invasion is still unclear. This study explores the role of NEAT1 in proliferation, migration, and invasion of trophoblasts. NEAT1 and miR-411-5p levels were detected by quantitative real-time PCR. Colony formation assay detected cell proliferation and transwell assay detected cell migration and invasion. Dual-luciferase reporter assay detected the binding between NEAT1 and miR-411-5p as well as the binding between miR-411-5p and PTEN. RNA pull-down assay detected the combination between NEAT1 and miR-411-5p. NEAT1 was increased and miR-411-5p was reduced in PE patients and human trophoblasts (HTR8/SVneo cells) that were induced with HO. Interference with NEAT1 promoted cell proliferation, migration, and invasion, and the miR-411-5p inhibitor reversed the effect of siRNA-NEAT1. The expression of PTEN was promoted in PE patients and HTR8/SVneo cells that were induced with HO, while the miR-411-5p mimic inhibited PTEN expression, and the plasmid-mediated PTEN overexpression reversed the effect of the miR-411-5p mimic. Besides, under HO induction, the miR-411-5p mimic promoted cell proliferation, migration, and invasion, and the plasmid-mediated PTEN overexpression reversed the effect of the miR-411-5p mimic. Interference with lncRNA NEAT1 promoted the proliferation, migration, and invasion of trophoblasts and alleviated the development of PE, which was partly mediated by upregulating miR-411-5p and inhibiting PTEN expression.
子痫前期 (PE) 是一种特发性妊娠高血压疾病，与异常胎盘绒毛发育有关。我们之前的研究发现，长链非编码 RNA NEAT1 促进滋养细胞凋亡，但 NEAT1 在增殖、迁移和侵袭中的作用尚不清楚。本研究探讨了 NEAT1 在滋养细胞增殖、迁移和侵袭中的作用。通过实时定量 PCR 检测 NEAT1 和 miR-411-5p 的水平。集落形成实验检测细胞增殖，transwell 实验检测细胞迁移和侵袭。双荧光素酶报告实验检测 NEAT1 与 miR-411-5p 的结合以及 miR-411-5p 与 PTEN 的结合。RNA 下拉实验检测 NEAT1 与 miR-411-5p 的结合。子痫前期患者和 HO 诱导的人滋养细胞 (HTR8/SVneo 细胞) 中 NEAT1 增加，miR-411-5p 减少。干扰 NEAT1 促进细胞增殖、迁移和侵袭，miR-411-5p 抑制剂逆转了 siRNA-NEAT1 的作用。HO 诱导的子痫前期患者和 HTR8/SVneo 细胞中 PTEN 表达增加，而 miR-411-5p 模拟物抑制 PTEN 表达，质粒介导的 PTEN 过表达逆转了 miR-411-5p 模拟物的作用。此外，在 HO 诱导下，miR-411-5p 模拟物促进细胞增殖、迁移和侵袭，质粒介导的 PTEN 过表达逆转了 miR-411-5p 模拟物的作用。干扰长链非编码 RNA NEAT1 促进滋养细胞的增殖、迁移和侵袭，缓解 PE 的发生发展，部分是通过上调 miR-411-5p 和抑制 PTEN 表达来实现的。
