急性脾脏照射对创伤性脑损伤大鼠的保护作用。

Protective effect of acute splenic irradiation in rats with traumatic brain injury.

机构信息

Department of Emergency.

Cancer Hospital, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China.

出版信息

Neuroreport. 2021 May 19;32(8):711-720. doi: 10.1097/WNR.0000000000001650.

DOI:10.1097/WNR.0000000000001650

PMID:33876783

Abstract

OBJECTIVE

To explore the protective effect of acute splenic irradiation against traumatic brain injury (TBI) in rats.

METHODS

A rat model of TBI was established according to Feeney's method. Splenic irradiation was performed by the reverse intensity-modulated radiation therapy (IMRT) source-axis distance (SAD) irradiation technique. Rat brain tissue samples were collected, the water content of the rat brain tissue was determined and the abundance of microglia was detected by immunofluorescence. Spleens were collected to measure the spleen index. Lung, liver, small intestine and kidney tissues were taken for hematoxylin and eosin staining to observe whether there was radiation-induced pathological damage. Peripheral blood was collected to detect tuftsin and the inflammatory factors IL-6 and IL-10.

RESULTS

Compared with the nonirradiated TBI rat group, the 4-h spleen irradiation TBI rat group showed (1) increased behavioral scores at 3 days after TBI (P < 0.05), (2) reduced water content of the ipsilateral hemisphere at 3 days after TBI, (3) reduced spleen index at 3 and 7 days after TBI, (4) reduced number of microglia cells infiltrating around the lesion at 7 days after TBI, (5) reduced IL-6 levels at 3 days after TBI, (6) increased IL-10 levels at 3 and 5days after TBI and (7) Compared with the nonirradiated TBI rat group, the 8-h spleen irradiation TBI rat group showed reduced tuftsin levels at 3 and 7days after TBI.

CONCLUSIONS

Acute splenic irradiation had a protective effect in rats with TBI.

摘要

目的

探讨急性脾照射对创伤性脑损伤（TBI）大鼠的保护作用。

方法

根据 Feeney 法建立 TBI 大鼠模型，采用反向调强放疗（IMRT）源轴距（SAD）照射技术进行脾照射。采集大鼠脑组织样本，测定大鼠脑组织含水量，免疫荧光法检测小胶质细胞丰度。采集脾脏，测量脾脏指数。取肺、肝、小肠和肾组织行苏木精和伊红染色，观察是否有放射性诱导的病理损伤。采集外周血，检测血栓生成素和炎症因子 IL-6 和 IL-10。

结果

与未照射 TBI 大鼠组相比，4 h 脾照射 TBI 大鼠组（1）TBI 后 3 天行为评分升高（P < 0.05），（2）TBI 后 3 天对侧半球含水量降低，（3）TBI 后 3、7 天脾脏指数降低，（4）TBI 后 7 天病变周围浸润的小胶质细胞数量减少，（5）TBI 后 3 天 IL-6 水平降低，（6）TBI 后 3、5 天 IL-10 水平升高，（7）与未照射 TBI 大鼠组相比，8 h 脾照射 TBI 大鼠组 TBI 后 3、7 天血栓生成素水平降低。