College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, Shandong, China.
Food Funct. 2021 May 21;12(10):4411-4423. doi: 10.1039/d1fo00286d. Epub 2021 Apr 20.
Alzheimer's disease (AD) is an age-dependent, irreversible neurodegenerative disease, and one of the pathological features is amyloid-β (Aβ) deposition. Previous studies have shown that phosphatidylserine (PS) enriched with eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) exhibited significant effects in preventing and alleviating the progress of AD. However, no studies have focused on the differences in the preventive effects on AD between EPA-PS and DHA-PS. Here, the effects of EPA-PS and DHA-PS on Aβ production, Aβ-induced neurotoxicity and Aβ clearance have been studied. The results show that DHA-PS significantly reduced Aβ production in CHO-APP/PS1 cells compared to EPA-PS. Moreover, both EPA-PS and DHA-PS significantly protected the primary hippocampal neurons against Aβ-induced toxicity by inhibiting the mitochondrial-dependent apoptotic pathway and phosphorylation of JNK and p38. Compared to DHA-PS, EPA-PS administration significantly improved the Aβ phagocytic capacity of BV2 cells. In addition, EPA-PS and DHA-PS significantly promoted the neurite outgrowth of primary hippocampal neurons. These findings might provide dietary guidance for the prevention of AD as well as a reference for the development of related functional foods.
阿尔茨海默病（AD）是一种年龄依赖性、不可逆转的神经退行性疾病，其病理特征之一是淀粉样β（Aβ）沉积。先前的研究表明，富含二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的磷脂酰丝氨酸（PS）在预防和缓解 AD 进展方面具有显著效果。然而，目前尚无研究关注 EPA-PS 和 DHA-PS 在预防 AD 方面的效果差异。本研究旨在探讨 EPA-PS 和 DHA-PS 对 Aβ产生、Aβ诱导的神经毒性和 Aβ清除的影响。结果表明，与 EPA-PS 相比，DHA-PS 可显著降低 CHO-APP/PS1 细胞中 Aβ的产生。此外，EPA-PS 和 DHA-PS 均可通过抑制线粒体依赖性凋亡途径和 JNK、p38 的磷酸化，显著保护原代海马神经元免受 Aβ诱导的毒性。与 DHA-PS 相比，EPA-PS 给药可显著提高 BV2 细胞对 Aβ的吞噬能力。此外，EPA-PS 和 DHA-PS 可显著促进原代海马神经元的突起生长。这些发现可能为 AD 的预防提供饮食指导，并为相关功能性食品的开发提供参考。
