Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, 100083, P. R. China.
Food Funct. 2021 Apr 7;12(7):3246-3265. doi: 10.1039/d0fo02975k. Epub 2021 Mar 23.
In this study, β-carotene loaded oil-in-water emulsions were stabilized by complex interfaces composed of propylene glycol alginate (PGA), rhamnolipids (Rha), and zein colloidal particles (ZCPs). The influence of mixed biopolymer-surfactant, biopolymer-particle, surfactant-particle and biopolymer-surfactant-particle interfaces on the performance of the emulsions was investigated. The stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of the emulsions were controlled by regulating the adding sequence and mass ratio of the multiple stabilizers. The droplet size of the emulsion was in the range of 14-77 μm. After encapsulation into the emulsions stabilized by the complex interfaces, the photothermal stability of β-carotene were increased by 41.53% and 21.52%, respectively. The co-existence of particles, biopolymers, and surfactants could induce competitive displacement, multilayer deposition and an interparticle network at the interface. Compared with a single PGA- or Rha-stabilized emulsion, the complex interface-stabilized emulsion reduced the release of FFA by 28.06% and 26.16%, respectively. The interfacial composition of the emulsion and the delayed lipid digestion further affected the bioaccessibility of β-carotene in the gastrointestinal tract (GIT). The mixed biopolymer-particle-surfactant interface-stabilized emulsion could be incorporated in foods, pharmaceuticals and cosmetics for excellent stability, targeted nutrient delivery and controlled lipolysis.
在这项研究中，β-胡萝卜素负载的油包水乳状液由由藻酸钠（PGA）、鼠李糖脂（Rha）和玉米醇溶蛋白胶体颗粒（ZCP）组成的复合界面稳定。考察了混合生物聚合物-表面活性剂、生物聚合物-颗粒、表面活性剂-颗粒和生物聚合物-表面活性剂-颗粒界面对乳液性能的影响。通过调节多种稳定剂的添加顺序和质量比来控制乳液的稳定性、微观结构、流变性能和体外胃肠道消化。乳液的粒径在 14-77 μm 范围内。在通过复合界面稳定的乳液中包封β-胡萝卜素后，其光热稳定性分别提高了 41.53%和 21.52%。颗粒、生物聚合物和表面活性剂的共存可以在界面处诱导竞争取代、多层沉积和颗粒间网络。与单一 PGA 或 Rha 稳定的乳液相比，复合界面稳定的乳液分别减少了 28.06%和 26.16%的游离脂肪酸释放。乳液的界面组成和延迟的脂质消化进一步影响了β-胡萝卜素在胃肠道（GIT）中的生物可及性。混合生物聚合物-颗粒-表面活性剂界面稳定的乳液可用于食品、药品和化妆品，以实现优异的稳定性、靶向营养传递和控制脂肪分解。
