掺锶磷酸钙骨水泥生物学性能的研究

[ study of strontium-doped calcium phosphate cement for biological properties].

作者信息

Wang J Q, Wang X

机构信息

Department of Stomatology, Peking University Third Hospital, Beijing 100191, China.

Department of Stomatology, the Second Affiliated Hospital of Hainan Medical University, Haikou 570311, China.

出版信息

Beijing Da Xue Xue Bao Yi Xue Ban. 2021 Mar 12;53(2):378-383. doi: 10.19723/j.issn.1671-167X.2021.02.024.

DOI:10.19723/j.issn.1671-167X.2021.02.024

PMID:33879914

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8072414/

Abstract

OBJECTIVE

To evaluate the biocompatibility and osteogenic effect of new calcium phosphate cement (CPC) and to provide experimental basis for its further clinical application.

METHODS

Thirty New Zealand white rabbits were randomly divided into four groups: CPC group, CPC+Bio-Oss group, Bio-Oss group and blank control group. Bone defect models of 6 mm in diameter and 7 mm in depth were made on the lateral condyle of bilateral hind legs of the rabbits. CPC, Bio-Oss and CPC+Bio-Oss mixture were implanted into the bone defect according to the group, and the mass ratio of CPC and Bio-Oss was 4 ∶ 1. The experimental animals were sacrificed the 4th, 12th and 24th week after operation. The tissue around the bone defect was taken for histological evaluation by H&E staining. Bone ingrowth fraction (BIF) was calculated. The expression of BMP-2 and COL-Ⅰ was detected by immunohis- tochemical staining by calculating the mean optical density (MOD) of the positive area the 4th week after operation, and the bone healing of each group was evaluated at different time points. The measurement data were analyzed by one-way ANOVA and LSD test was used for multiple comparison of the differences between the means by SPSS 19.0. < 0.05 was considered to be statistically significant.

RESULTS

The results of H&E staining showed that the BIF values of CPC group, CPC + Bio-Oss group and Bio-Oss group were significantly higher than those of blank control group at the same time point ( < 0.01). The BIF values of CPC group were lower than those of Bio-Oss group and CPC + Bio-Oss group ( < 0.01). There was no significant difference between CPC + Bio-Oss group and Bio-Oss group. Immunohistochemical staining showed that the MOD values of BMP-2 and COL-Ⅰ in CPC group were higher than those in blank control group, but lower than those in Bio-Oss group and CPC+Bio-Oss group ( < 0.01). There was no significant difference between BMP-2 and COL-Ⅰ in CPC+Bio-Oss group and Bio-Oss group.

CONCLUSION

The new calcium phosphate cement has good biocompatibility and can promote early osteogenesis with stable and long-term effect.

摘要

目的

评价新型磷酸钙骨水泥（CPC）的生物相容性和成骨效果，为其进一步临床应用提供实验依据。

方法

将30只新西兰白兔随机分为四组：CPC组、CPC+Bio-Oss组、Bio-Oss组和空白对照组。在兔双侧后肢外侧髁制作直径6 mm、深7 mm的骨缺损模型。按组分别将CPC、Bio-Oss及CPC+Bio-Oss混合物植入骨缺损处，CPC与Bio-Oss的质量比为4∶1。术后第4、12和24周处死实验动物。取骨缺损周围组织行苏木精-伊红（H&E）染色进行组织学评价，计算骨长入分数（BIF）。术后第4周通过免疫组织化学染色检测骨形态发生蛋白-2（BMP-2）和Ⅰ型胶原蛋白（COL-Ⅰ）的表达，计算阳性区域的平均光密度（MOD），并在不同时间点评估各组的骨愈合情况。计量资料采用单因素方差分析，用SPSS 19.0软件进行均数间多重比较的LSD检验。P<0.05认为差异有统计学意义。

结果

H&E染色结果显示，同一时间点CPC组、CPC+Bio-Oss组和Bio-Oss组的BIF值均显著高于空白对照组（P<0.01）。CPC组的BIF值低于Bio-Oss组和CPC+Bio-Oss组（P<0.01）。CPC+Bio-Oss组与Bio-Oss组之间差异无统计学意义。免疫组织化学染色显示，CPC组BMP-2和COL-Ⅰ的MOD值高于空白对照组，但低于Bio-Oss组和CPC+Bio-Oss组（P<0.01）。CPC+Bio-Oss组与Bio-Oss组BMP-2和COL-Ⅰ之间差异无统计学意义。