Department of Chemistry, UCS, Osmania University, Hyderabad, Telangana State, 500007, India.
Department of Chemistry, Government Degree College for Women, Karimnagar, Telangana State, 505001, India.
J Fluoresc. 2021 Jul;31(4):1009-1021. doi: 10.1007/s10895-021-02727-y. Epub 2021 Apr 20.
A new ligand FIPB = 5-(1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl)furan-2-yl-2-boronic acid, having three cobalt(III) polypyridyl complexes Co(phen)(FIPB) {FIPB = 5-(1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl)furan-2-yl-2-boronic acid}, (phen = 1,10-Phenanthroline), Co(bpy)(FIPB) (bpy = 2,2'bipyridyl), Co(dmb)(FIPB) (dmb = 4, 4'-dimethyl 2, 2'-bipyridine) have been synthesized and characterized by elemental analysis, ES-MS,H-NMR, C-NMR, UV-Vis and FTIR. Their DNA binding behavior has been explored by various spectroscopic titrations and viscosity measurements, which indicated that all the complexes bind to calf thymus DNA by means of intercalation with different binding strengths. The binding properties of these all three complexes towards calf-thymus DNA (CT-DNA) have been investigated by UV-visible, emission spectroscopy and viscosity measurements.The experimental results suggested that three Co(III) complexes can intercalate into DNA base pairs,but with different binding affinities. Photo induced DNA cleavage studies have been performed and results indicate that three complexes efficiently cleave the pBR322-DNA in different forms. The three synthesized compounds were tested for antimicrobial activity by using Staphylococcus aureus and Bacillus subtilis organisms, these results indicated that complex 1 was more activity compared to other two complexes against both tested microbial strains. The in vitro cytotoxicity of these complexes was evaluatedby MTT assay, and complex 1 shows higher cytotoxicity than complex 2 and 3 on HeLa cells.
一种新的配体 FIPB = 5-(1H-咪唑并[4,5-f][1,10]菲咯啉-2-基)呋喃-2-基-2-硼酸，具有三个钴(III)多吡啶配合物 [Co(phen)(FIPB)]（1）{FIPB = 5-(1H-咪唑并[4,5-f][1,10]菲咯啉-2-基)呋喃-2-基-2-硼酸}，（phen = 1,10-菲咯啉），[Co(bpy)(FIPB)]（2）（bpy = 2,2'-联吡啶），[Co(dmb)(FIPB)]（3）（dmb = 4,4'-二甲基 2,2'-联吡啶）已被合成并通过元素分析、ES-MS、H-NMR、C-NMR、UV-Vis 和 FTIR 进行了表征。通过各种光谱滴定和粘度测量研究了它们的 DNA 结合行为，表明所有配合物都通过嵌入与不同结合强度与小牛胸腺 DNA 结合。通过紫外可见光谱、荧光光谱和粘度测量研究了这三种配合物与小牛胸腺 DNA（CT-DNA）的结合特性。实验结果表明，三种 Co(III)配合物可以嵌入 DNA 碱基对，但结合亲和力不同。进行了光诱导 DNA 切割研究，结果表明三种配合物可以以不同的形式有效地切割 pBR322-DNA。三种合成的化合物通过金黄色葡萄球菌和枯草芽孢杆菌进行了抗菌活性测试，结果表明，与其他两种配合物相比，配合物 1 对两种测试的微生物菌株更具活性。通过 MTT 测定法评估了这些配合物的体外细胞毒性，配合物 1 在 HeLa 细胞上显示出比配合物 2 和 3 更高的细胞毒性。
