Department of Biosciences, Cancer Genetics Lab, COMSATS University Islamabad, Islamabad, 44000, Pakistan.
Department of Molecular Biology, Shaheed Zulfiqar Ali Bhutto Medical University, Islamabad, 44000, Pakistan.
Future Oncol. 2021 Jul;17(21):2725-2734. doi: 10.2217/fon-2020-1080. Epub 2021 Apr 21.
plays a key role as a transcriptional coactivator in the Hippo pathway. Based on conflicting reports regarding function in cancer, this study discerned its role in breast carcinogenesis. First, a systematic review of salient breast cancer studies targeting YAP1 dysregulation was performed. Additionally, freshly excised tumor specimens of approximately 200 breast cancer patients were processed for quantification of expression at mRNA and protein levels using quantitative PCR and immunohistochemistry, respectively. expression was nine folds higher in tumors versus controls and significantly associated with metastasis (p < 0.05) and poor survival in Pakistani breast cancer patients. These findings establish the role of overexpression in tumorigenesis and metastasis. Hence, inhibition may be considered a possible therapeutic strategy.
在 Hippo 通路中作为转录共激活因子发挥关键作用。基于关于其在癌症中的功能的相互矛盾的报告，本研究探讨了其在乳腺癌发生中的作用。首先，对针对 YAP1 失调的有意义的乳腺癌研究进行了系统回顾。此外，使用定量 PCR 和免疫组织化学分别对大约 200 例乳腺癌患者的新鲜切除肿瘤标本进行了定量分析，以检测 表达的 mRNA 和蛋白水平。与对照组相比，肿瘤中的 表达高出九倍，并且与转移（p < 0.05）和巴基斯坦乳腺癌患者的不良生存显著相关。这些发现确立了 过表达在肿瘤发生和转移中的作用。因此，抑制 可能被认为是一种可行的治疗策略。
