Department of Chemistry, University of Bari, via Orabona, 4, 70125, Bari, Italy.
Curr Opin Chem Biol. 2021 Apr;61:214-226. doi: 10.1016/j.cbpa.2021.03.003. Epub 2021 Apr 18.
Metal-based drugs can modulate various biological processes and exhibit a rich variety of properties that foster their use in biomedicine and chemical biology. On the way to intracellular targets, ligand exchange and redox reactions can take place, thus making metallodrug speciation in vivo a challenging task. Advances in NMR spectroscopy have made it possible to move from solution to live-cell studies and elucidate the transport of metallodrugs and interactions with macromolecular targets in a physiological setting. In turn, the electronic properties and supramolecular chemistry of metal complexes can be exploited to characterize drug delivery nanosystems by NMR. The recent evolution of in-cell NMR methodology is presented with special emphasis on metal-related processes. Applications to paradigmatic cases of platinum and gold drugs are highlighted.
金属基药物可以调节各种生物过程，并表现出丰富多样的性质，这促进了它们在生物医学和化学生物学中的应用。在向细胞内靶标移动的过程中，可能会发生配体交换和氧化还原反应，因此使得金属药物在体内的形态成为一项具有挑战性的任务。NMR 光谱学的进步使得从溶液到活细胞研究成为可能，并阐明了金属药物的转运及其在生理环境下与生物大分子靶标的相互作用。反过来，金属配合物的电子性质和超分子化学可以通过 NMR 来表征药物传递纳米系统。本文特别强调了与金属相关的过程，介绍了细胞内 NMR 方法学的最新进展。还突出了应用于典型的铂类和金类药物的案例。
