使用乳胶颗粒对抗原和抗体进行比浊测定。
Nephelometric assay of antigens and antibodies with latex particles.
作者信息
Grange J, Roch A M, Quash G A
出版信息
J Immunol Methods. 1977;18(3-4):365-75. doi: 10.1016/0022-1759(77)90190-9.
PMID:338836
Abstract
Antigens and antibodies covalently bound to latex particles have been used to detect specifically corresponding antibodies and antigens by nephelometry. A systematic study of factors such as wavelength, angle of observation, concentration of latex particles, and reaction time has permitted us to develop a procedure suitable for routine estimation of antigens and antibodies in the nano and picogramme range.
摘要
与乳胶颗粒共价结合的抗原和抗体已被用于通过比浊法特异性检测相应的抗体和抗原。对诸如波长、观察角度、乳胶颗粒浓度和反应时间等因素进行的系统研究，使我们能够开发出一种适用于常规估算纳克和皮克范围内抗原和抗体的方法。
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