School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
World J Microbiol Biotechnol. 2021 Apr 22;37(5):89. doi: 10.1007/s11274-021-03055-w.
Coumarin is widely used in personal care products and pharmaceutical industry, which leads to the release of this compound into environment as an emerging contaminant. Here, a promising strain USTB-Z for biodegrading coumarin was successfully isolated from botanical soil and characterized as a potential novel Pseudomonas sp. based on 16S rDNA sequence analysis and orthologous average nucleotide identity tool. Initial coumarin up to 800 mg/L could be completely removed by USTB-Z within 48 h at the optimal culture conditions of pH 7.3 and 30 °C, which indicates that USTB-Z has a strong capacity in coumarin biodegradation. The biodegradation products of coumarin were further investigated using HPLC and Q-TOF LC/MS, and melilotic acid and 2,3-dihydroxyphenylpropionic acid were identified. The draft genome of strain USTB-Z was sequenced by Illumina NovaSeq, and 21 CDSs for NAD (P)-dependent oxidoreductase, 43 CDSs for hydrolase, 1 CDS for FAD-depend monooxygenase, 1 CDS for 3-hydroxycinnamic acid hydroxylase, 21 CDSs for dioxygenase, and 5 CDSs for fumarylacetoacetate (FAA) hydrolase were annotated and correlated to coumarin biodegradation. The present study provides a theoretical basis and microbial resource for further research on the coumarin biodegradation.
香豆素广泛应用于个人护理产品和制药行业，这导致这种化合物作为一种新兴污染物释放到环境中。在这里，我们成功地从植物土壤中分离到一株有潜力的香豆素降解菌 USTB-Z，并根据 16S rDNA 序列分析和直系同源平均核苷酸同一性工具将其鉴定为一种潜在的新型假单胞菌。在最适培养条件 pH 7.3 和 30°C 下，USTB-Z 可以在 48 h 内完全去除高达 800 mg/L 的初始香豆素，这表明 USTB-Z 具有很强的香豆素生物降解能力。进一步使用 HPLC 和 Q-TOF LC/MS 研究了香豆素的生物降解产物，鉴定出了蜜二糖酸和 2,3-二羟苯丙酸。采用 Illumina NovaSeq 对菌株 USTB-Z 的基因组进行了测序，共注释了 21 个 NAD(P)-依赖性氧化还原酶 CDS、43 个水解酶 CDS、1 个 FAD 依赖性单加氧酶 CDS、1 个 3-羟基肉桂酸羟化酶 CDS、21 个双加氧酶 CDS 和 5 个富马酰乙酰乙酸（FAA）水解酶 CDS，这些基因与香豆素的生物降解有关。本研究为进一步研究香豆素的生物降解提供了理论基础和微生物资源。
