Faculty of Land Resource Engineering, Kunming University of Science and Technology, Kunming 650500, China.
Shenzhen Institute of Respiratory Diseases, Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, The First Affiliated Hospital (Shenzhen People's Hospital), Southern University of Science and Technology, Shenzhen 518000, China.
Anal Methods. 2021 Apr 22;13(15):1810-1815. doi: 10.1039/d0ay02213f.
Microbial biomass, as an environmentally friendly resource, has attracted considerable attention as a green biomaterial for the production of unique and functionalised CDs; however, further exploration is required to characterise CDs derived from bacteria. In this study, a green biomaterial (fluorescence CDs-HS18) was successfully synthesised via a hydrothermal method from Ureibacillus thermosphaericus HS-18 specimens isolated from a hot spring. The prepared CDs-HS18 possess excellent photo-physical properties, outstanding fluorescence capabilities, and high biocompatibility, which make them desirable candidates for multi-mode imaging applications. Our results demonstrate that the prepared CDs can selectively stain the membrane of the biological cells tested and can be rapidly distributed to all parts of the leaf via the veins and intercellular interstitium through transpiration. Additionally, CDs-HS18 are likely to enter the digestive tract of Microworms through ingestion and spread rapidly through the entire body and may finally be excreted through the anus. Furthermore, the rapid and highly selective detection platform based on CDs-HS18 exhibited an excellent linear response for Cr6+ between 0 and 9 μM, with a detection limit of 36 nM. This research will expand the understanding of the characteristics of green biomaterials derived from bacteria and widen the application scope of hot spring resources.
微生物生物量作为一种环保资源，作为一种生产独特和功能化碳点的绿色生物材料引起了相当大的关注；然而，需要进一步探索来表征细菌衍生的碳点。在这项研究中，通过从温泉中分离出的 Ureibacillus thermosphaericus HS-18 样本，通过水热法成功合成了一种绿色生物材料（荧光碳点-HS18）。所制备的碳点-HS18 具有优异的光物理性质、出色的荧光性能和高生物相容性，使其成为多模式成像应用的理想候选材料。我们的结果表明，所制备的碳点可以选择性地染色测试的细胞膜，并通过蒸腾作用通过叶脉和细胞间隙迅速分布到叶子的各个部位。此外，碳点-HS18 可能通过摄入进入 Microworms 的消化道，并迅速分布到全身，最终可能通过肛门排出。此外，基于碳点-HS18 的快速和高选择性检测平台对 0 到 9 μM 之间的 Cr6+ 表现出优异的线性响应，检测限为 36 nM。这项研究将扩展对细菌衍生绿色生物材料特性的理解，并拓宽温泉资源的应用范围。
