脑转录组分析揭示了参与盘鱼（Symphysodon haraldi）亲代照顾行为的基因。
Brain transcriptome analysis reveals genes involved in parental care behaviour in discus fish (Symphysodon haraldi).
机构信息
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China.
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China.
出版信息
Gen Comp Endocrinol. 2021 Aug 1;309:113793. doi: 10.1016/j.ygcen.2021.113793. Epub 2021 Apr 19.
Parental care is common in mammals and allows offspring to obtain milk, a substance rich in a range of nutritional and non-nutritional factors crucial to the survival of newborns. The discus fish Symphysodon spp., an Amazonian cichlid, shows an unusual behaviour: Free-swimming fry bite on their parents' skin mucus for growth and development during the first month after hatching. This is similar to the breastfeeding behaviour of mammals, but little is known about the regulatory mechanism by which discus secrete 'milk' and the related genes involved in parental care. Here, transcriptome sequencing was performed by using the brain tissues of female discus fish in parental and non-parental care. The results showed that a total of 86 differentially expressed genes (71 up-regulated genes and 15 down-regulated genes) were obtained by comparing parental with non-parental discus fish, including up-regulated LAPTM, FOXB, SOX1S, OTX2 and NR1F2, and down-regulated EDNRB, PRKCD, H1-5 and HBE. Through functional enrichment analysis, a total of 20 pathways were identified, e.g., estrogen signaling pathway, inflammatory mediator regulation of TRP channels, vascular smooth muscle contraction, GnRH signaling pathway, neurotrophin signaling pathway, NOD-like receptor signaling pathway, Jak-STAT signaling pathway, Fc gamma R-mediated phagocytosis, serotonergic synapse, autophagy-animal and cytokine-cytokine receptor interaction. These pathways and related genes might play important roles in the regulation of discus 'milk' secretion.
亲代照顾在哺乳动物中很常见，它使后代能够获得富含各种营养和非营养因素的乳汁，这些因素对新生儿的生存至关重要。亚马逊慈鲷盘丽鱼属的神仙鱼表现出一种不寻常的行为：在孵化后第一个月的自由游动阶段，幼鱼会咬父母的皮肤黏液来获取生长和发育所需的物质。这类似于哺乳动物的母乳喂养行为，但对于神仙鱼分泌“乳汁”的调节机制以及涉及亲代照顾的相关基因知之甚少。在这里，通过对处于亲代照顾和非亲代照顾状态的雌鱼脑组织进行转录组测序。结果表明，通过比较亲代和非亲代神仙鱼，共获得了 86 个差异表达基因（71 个上调基因和 15 个下调基因），包括上调的 LAPTM、FOXB、SOX1S、OTX2 和 NR1F2，以及下调的 EDNRB、PRKCD、H1-5 和 HBE。通过功能富集分析，共鉴定出 20 条通路，如雌激素信号通路、TRP 通道炎症介质调节、血管平滑肌收缩、GnRH 信号通路、神经营养素信号通路、NOD 样受体信号通路、Jak-STAT 信号通路、FcγR 介导的吞噬作用、5-羟色胺能突触、自噬-动物和细胞因子-细胞因子受体相互作用。这些通路和相关基因可能在调节神仙鱼“乳汁”分泌中发挥重要作用。
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