全基因组鉴定及七彩神仙鱼（Symphysodon aequifasciatus）免疫球蛋白超家族的皮肤表达揭示了与脊椎动物泌乳相关的共同基因。
Genome-wide identification and skin expression of immunoglobulin superfamily in discus fish (Symphysodon aequifasciatus) reveal common genes associated with vertebrate lactation.
作者信息
Liu Xin, Ding Xiang-Fei, Wen Bin, Ma Teng-Fei, Li Zhong-Jun, Zhang Yan-Shen, Gao Jian-Zhong, Chen Zai-Zhong
机构信息
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China; National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China.
出版信息
Gene. 2023 Apr 30;862:147260. doi: 10.1016/j.gene.2023.147260. Epub 2023 Feb 10.
Discus Symphysodon spp. employs an unusual parental care behavior where fry feed on parental skin mucus after hatching. Studies on discus immunoglobulin superfamily (IgSF) especially during parental care are scarce. Here, a total of 518 IgSF members were identified based on discus genome and clustered into 12 groups, unevenly distributing on 30 linkage groups. A total of 92 pairs of tandem duplication and 40 pairs of segmental duplication that underwent purifying selection were identified. IgSF genes expressed differentially in discus skin during different care stages and between male and female parents. Specifically, the transcription of btn1a1, similar with mammalian lactation, increased after spawning, reached a peak when fry started biting on parents' skin mucus, and then decreased. The expression of btn2a1 and other immune members, e.g., nect4, fcl5 and cd22, were up-regulated when fry stopped biting on mucus. These results suggest the expression differentiation of IgSF genes in skin of discus fish during parental care.
七彩神仙鱼（盘丽鱼属物种）具有一种独特的亲代抚育行为，即幼鱼在孵化后以亲鱼的皮肤黏液为食。关于七彩神仙鱼免疫球蛋白超家族（IgSF）的研究，尤其是在亲代抚育期间的研究很少。在此，基于七彩神仙鱼的基因组共鉴定出518个IgSF成员，并将其聚类为12个组，不均匀地分布在30个连锁群上。共鉴定出92对串联重复和40对经历纯化选择的片段重复。IgSF基因在不同抚育阶段以及雌雄亲鱼的皮肤中差异表达。具体而言，与哺乳动物泌乳相似的btn1a1在产卵后转录增加，在幼鱼开始啃咬亲鱼皮肤黏液时达到峰值，然后下降。当幼鱼停止啃咬黏液时，btn2a1和其他免疫成员（如nect4、fcl5和cd22）的表达上调。这些结果表明在亲代抚育期间七彩神仙鱼皮肤中IgSF基因的表达分化。
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