Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Gene. 2021 Jul 1;788:145674. doi: 10.1016/j.gene.2021.145674. Epub 2021 Apr 20.
During the growing season of 2018, several field-grown cucurbit plants in different parts of Iraq and Iran were surveyed for the presence of zucchini yellow mosaic virus (ZYMV), using two degenerate primer pairs (CIF/Rev and NIb2F/3R) targeting the two separated partial regions of the potyvirus genome (CI and NIb respectively). 7 out of 20 samples were confirmed to be infected with ZYMV. Phylogenetic analyses based on the CI gene grouped all Iranian and two Iraqi (ZYMV1 and ZYMV2) isolates together with isolates from the Middle East in the subgroup (AI), whereas the other Iraqi (ZYMV3 and ZYMV4) isolates were clustered in the subgroup (DI), which was only consisted of American isolates. The highest and lowest identity between the studied isolates and the GenBank isolates showed that the two genes (CI, NIb) of each isolate particularly the Iraqi isolates were more similar to a specific and geographically scattered mosaic of worldwide isolates, suggestive of mixed infection might have occurred between different worldwide isolates in Iraq. Furthermore, the first complete nucleotide sequence of an Iraqi ZYMV (ZYMV-Iq) isolate was done, using the Illumina sequencing technique. The complete nucleotide sequence of ZYMV-Iq isolate was 9650 nt, excluding the 3'poly (A) tail. ZYMV-Iq isolate shared the highest nt identity of 98.8% with an American (KC665630) isolate. Phylogenetic analysis based on the full genome sequence placed ZYMV-Iq in subgroup A of group I alongside 18 isolates from the US and two isolates from Australia. In addition, recombination analysis detected lone significant recombination between ZYMV-Iq and South Korean (AY279000) isolate. Moreover, the results showed that symptom intensity was varied across experimental host plants.
在 2018 年的生长季节，在伊拉克和伊朗不同地区对几种田间生长的葫芦科植物进行了调查，以检测是否存在南瓜黄花叶病毒（ZYMV），使用了两对针对马铃薯 Y 病毒基因组两个分离的部分区域（CI 和 NIb 分别）的简并引物对（CIF/Rev 和 NIb2F/3R）。20 个样本中有 7 个被确认为感染了 ZYMV。基于 CI 基因的系统发育分析将所有伊朗和两个伊拉克（ZYMV1 和 ZYMV2）分离株与中东分离株一起分组在亚组（AI）中，而其他两个伊拉克（ZYMV3 和 ZYMV4）分离株则聚类在亚组（DI）中，该亚组仅由美国分离株组成。研究的分离株与 GenBank 分离株之间的最高和最低同一性表明，每个分离株的两个基因（CI、NIb），特别是伊拉克分离株，与全球分离株中特定且地理上分散的马赛克更为相似，表明在伊拉克，不同的全球分离株之间可能发生了混合感染。此外，还使用 Illumina 测序技术完成了伊拉克 ZYMV（ZYMV-Iq）分离株的全长核苷酸序列。ZYMV-Iq 分离株的全长核苷酸序列为 9650nt，不包括 3'poly（A）尾。ZYMV-Iq 分离株与美国（KC665630）分离株的核苷酸同一性最高，为 98.8%。基于全基因组序列的系统发育分析将 ZYMV-Iq 分离株置于 I 组的 A 亚组中，与来自美国的 18 个分离株和来自澳大利亚的 2 个分离株并列。此外，重组分析检测到 ZYMV-Iq 与韩国（AY279000）分离株之间存在单独的显著重组。此外，结果表明，症状强度在不同的实验宿主植物之间有所不同。
