Marzachí C, Milne R G, Boccardo G
Istituto di Fitovirologia applicata del C.N.R., Torino, Italy.
Virology. 1988 Jul;165(1):115-21. doi: 10.1016/0042-6822(88)90664-2.
Partially purified carnation cryptic virus (CarCV) preparations possessed RNA-dependent RNA polymerase activity which was absent in comparable preparations from virus-free carnations. Enzyme activity was dependent upon the presence of virus particles, Mg2+, and the four ribonucleoside triphosphates, and was insensitive to inhibitors of DNA-dependent RNA polymerases. The 32P-labeled enzyme reaction products were largely dsRNAs as indicated by resistance to S1 nuclease and RNase A at high but not low ionic strength. The in vitro synthesized dsRNAs hybridized specifically with CarCV genomic dsRNAs, and the radioactive products present in the polymerase reaction mixture sedimented with the virus particles in sucrose density gradients. The data suggest that the RNA-dependent RNA polymerase associated with CarCV particles is a replicase which catalyzes the synthesis of copies of the genomic dsRNAs.
部分纯化的香石竹潜隐病毒（CarCV）制剂具有RNA依赖的RNA聚合酶活性，而来自无病毒香石竹的类似制剂中则不存在这种活性。酶活性依赖于病毒颗粒、Mg2+和四种核糖核苷三磷酸的存在，并且对DNA依赖的RNA聚合酶抑制剂不敏感。32P标记的酶反应产物在高离子强度而非低离子强度下对S1核酸酶和核糖核酸酶A具有抗性，这表明其主要为双链RNA。体外合成的双链RNA与CarCV基因组双链RNA特异性杂交，并且聚合酶反应混合物中存在的放射性产物在蔗糖密度梯度中与病毒颗粒一起沉降。这些数据表明，与CarCV颗粒相关的RNA依赖的RNA聚合酶是一种复制酶，可催化基因组双链RNA的拷贝合成。
