登革2型病毒牙买加基因型非结构蛋白的核苷酸序列及推导的氨基酸序列：全长基因组的比较分析

Nucleotide sequence and deduced amino acid sequence of the nonstructural proteins of dengue type 2 virus, Jamaica genotype: comparative analysis of the full-length genome.

作者信息

Deubel V, Kinney R M, Trent D W

机构信息

Division of Vector-Borne Viral Diseases, Centers for Disease Control, Fort Collins, Colorado 80522.

出版信息

Virology. 1988 Jul;165(1):234-44. doi: 10.1016/0042-6822(88)90677-0.

DOI:10.1016/0042-6822(88)90677-0

PMID:3388770

Abstract

The sequence of the 5'-end of the genome of dengue 2 (Jamaica genotype) virus has been previously reported (V. Deubel, R. M. Kinney, and D. W. Trent, 1986, Virology 155, 365-377). We have now cloned and sequenced the remaining 75% of the genomic RNA that encodes the nonstructural proteins. The complete genome is 10,723 bases in length with a single open reading frame extending from nucleotides 97 to 10,269 encoding 3391 amino acids. The 3'-noncoding extremity presents a stem- and loop-structure and contains a repeated oligonucleotide sequence. Comparisons of the nucleotide sequences of the genomes of dengue 2 viruses of different topotypes reveal 90-95% similarity, with 64-66% similarity evident between dengue viruses of different serotypes. The amino acid sequence of the polyprotein of dengue 2 Jamaica virus shows 97, 68, 50, and 44% similarity with those of other dengue 2, dengue 1, or dengue 4, West Nile, and yellow fever viruses, respectively. Despite amino acid sequence divergence, the hydrophobic profile of the flavivirus proteins is highly conserved. Proteins NS1, NS3, and NS5 are the most conserved. Conserved amino acid stretches present in all flavivirus proteins may be involved in common essential biological functions.

摘要

登革2型（牙买加基因型）病毒基因组5'端的序列先前已有报道（V. 德贝尔、R. M. 金尼和D. W. 特伦特，1986年，《病毒学》155卷，第365 - 377页）。我们现已克隆并测序了编码非结构蛋白的基因组RNA的其余75%。完整基因组长度为10,723个碱基，有一个单一的开放阅读框，从核苷酸97延伸至10,269，编码3391个氨基酸。3'非编码末端呈现出茎环结构，并含有一个重复的寡核苷酸序列。不同拓扑型的登革2型病毒基因组核苷酸序列比较显示相似性为90 - 95%，不同血清型的登革病毒之间的相似性为64 - 66%。登革2型牙买加病毒多聚蛋白的氨基酸序列与其他登革2型、登革1型、登革4型、西尼罗河病毒和黄热病病毒的氨基酸序列分别显示出97%、68%、50%和44%的相似性。尽管氨基酸序列存在差异，但黄病毒属蛋白质的疏水特性高度保守。非结构蛋白1（NS1）、非结构蛋白3（NS3）和非结构蛋白5（NS5）是最保守的。所有黄病毒属蛋白质中存在的保守氨基酸序列片段可能参与共同的基本生物学功能。