Department of Biochemistry and Pharmacology, Kirkuk University College of Veterinary Medicine, Kirkuk, Iraq.
Antiinflamm Antiallergy Agents Med Chem. 2021;20(4):373-379. doi: 10.2174/1871523020666210422132618.
Polycystic Ovary Syndrome (PCOS) is the most common endocrine disorder in women of reproductive age. PCOS is a heterogeneous complex disorder of defined aetiology. Some studies report its association with various endocrine, metabolic and immunological abnormalities. The hunger hormones ghrelin and leptin affect the pathogenesis of PCOS and might lead to the development of Metabolic Syndrome (MS) in obese women.
The study aims at evaluating the role of ghrelin and leptin level in females with polycystic ovary syndrome as a biochemical marker for the diagnosis and monitoring progression.
The study included one hundred PCOS patients and fifty apparently healthy subjects with regular menstrual cycle, visiting gynecology outpatient clinic of Kalar General Hospital, from the beginning of February 2015 to the end of June 2015. Body Mass Index (BMI) along with serum ghrelin, leptin, Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH) and testosterone levels were measured for both groups. Serum leptin was determined using sandwich ELISA, while serum ghrelin was determined using competitive ELISA. Differences between patient and control groups were assessed by using a t-test. Also, one-way Anova was used to test the relationships among different groups.
A significant decrease in ghrelin level and an increase in leptin levels in women with PCOS were observed compared to the control group. Also, a significant elevation in serum levels of LH, Testosterone, Prolactin, and a decrease in serum FSH in PCOS patients were observed, when compared to the control group. Additionally, serum ghrelin decreased and serum leptin level increased significantly in women with PCOS compared to controls in all age and BMI groups.
A significant decrease in ghrelin and an increase in leptin in PCOS patients were observed than in controls, indicating that they are at high risk for metabolic syndrome development.
多囊卵巢综合征（PCOS）是育龄妇女中最常见的内分泌疾病。PCOS 是一种病因明确的异质性复杂疾病。一些研究报告称，它与各种内分泌、代谢和免疫异常有关。饥饿激素 ghrelin 和 leptin 影响 PCOS 的发病机制，并可能导致肥胖女性代谢综合征（MS）的发展。
本研究旨在评估 ghrelin 和 leptin 水平在多囊卵巢综合征女性中的作用，作为诊断和监测进展的生化标志物。
本研究纳入了 2015 年 2 月初至 6 月底在 Kalar 综合医院妇科门诊就诊的 100 例 PCOS 患者和 50 例月经周期正常的健康对照者。测量两组患者的体质指数（BMI）以及血清 ghrelin、leptin、黄体生成素（LH）、卵泡刺激素（FSH）和睾酮水平。使用夹心 ELISA 法测定血清 leptin，使用竞争 ELISA 法测定血清 ghrelin。采用 t 检验比较患者组和对照组之间的差异。采用单因素方差分析检验不同组之间的关系。
与对照组相比，PCOS 患者的 ghrelin 水平显著降低，leptin 水平升高。与对照组相比，PCOS 患者血清 LH、Testosterone、Prolactin 水平显著升高，血清 FSH 水平降低。此外，与对照组相比，所有年龄和 BMI 组的 PCOS 患者血清 ghrelin 降低，血清 leptin 水平升高。
与对照组相比，PCOS 患者的 ghrelin 显著降低，leptin 显著升高，表明她们发生代谢综合征的风险较高。
