Lychko A P, Bogdanov N G, Pentiuk A A, Lutsiuk N B
Vopr Pitan. 1988 Mar-Apr(2):38-41.
Vitamin B1 in rats induced in the liver elevation of activity of metabolic enzymes of xenobiotics bound with membranes (dimethylaniline N-demethylase, aniline n-hydroxylase, aryl esterase). At the same time activity of the cytoplasmatic enzymes alcohol dehydrogenase and glutathione-S-transferase was appreciably lowered. An additional load with thiamine (20 mg/kg) led to a drop in activity of the membrane-bound enzymes. Vitamin B1 deficiency modified the effect of the inductor phenobarbital. Additional administration of vitamin B1 to thiamine-deficient animals normalized the thiamine level in the liver, and activity of hydroxylase, aryl esterase, formaldehyde dehydrogenase, and significantly decreased demethylase activity. In vitamin B1-deficient animals high detergent concentrations significantly suppressed NADH-dichlorophenol-indophenol-reductase activity, while low concentrations activated this enzyme as compared to the control.
大鼠体内的维生素B1会使肝脏中与膜结合的外源性物质代谢酶（二甲基苯胺N-脱甲基酶、苯胺N-羟化酶、芳基酯酶）的活性升高。与此同时，细胞质酶乙醇脱氢酶和谷胱甘肽-S-转移酶的活性显著降低。额外补充硫胺素（20毫克/千克）会导致膜结合酶的活性下降。维生素B1缺乏改变了诱导剂苯巴比妥的作用。给硫胺素缺乏的动物额外补充维生素B1可使肝脏中的硫胺素水平正常化，同时羟化酶、芳基酯酶、甲醛脱氢酶的活性恢复正常，脱甲基酶的活性显著降低。在维生素B1缺乏的动物中，高浓度去污剂会显著抑制NADH-二氯酚靛酚还原酶的活性，而低浓度去污剂与对照组相比则会激活该酶。
