Zhang Shasha, Dong Ying, Qiang Ruiying, Zhang Yuan, Zhang Xiaoli, Chen Yin, Jiang Pei, Ma Xiangyu, Wu Leilei, Ai Jingru, Gao Xia, Wang Pengjun, Chen Jie, Chai Renjie
State Key Laboratory of Bioelectronics, School of Life Sciences and Technology, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China.
Jiangsu Provincial Key Medical Discipline (Laboratory), Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.
Front Genet. 2021 Apr 6;12:625867. doi: 10.3389/fgene.2021.625867. eCollection 2021.
Striatin-interacting protein 1 () is a core component of the striatin interacting phosphatase and kinase (STRIPAK) complex, which is involved in embryogenesis and development, circadian rhythms, type 2 diabetes, and cancer progression. However, the expression and role of in the mammalian cochlea remains unclear. Here we studied the expression and function of in the mouse cochlea by using knockout mice. We first found that the mRNA and protein expression of increases as mice age starting from postnatal day (P) 3 and reaches its highest expression level at P30 and that the expression of can be detected by immunofluorescent staining starting from P14 only in cochlear HCs, and not in supporting cells (SCs). Next, we crossed heterozygous knockout ( +/-) mice to obtain homozygous knockout (-/-) mice for studying the role of in cochlear HCs. However, no -/- mice were obtained and the ratio of +/- to +/+ mice per litter was about 2:1, which suggested that homozygous knockout is embryonic lethal. We measured hearing function and counted the HC number in P30 and P60 +/- mice and found that they had normal hearing ability and HC numbers compared to +/+ mice. Our study suggested that probably play important roles in HC development and maturation, which needs further study in the future.
striatin相互作用蛋白1（）是striatin相互作用磷酸酶和激酶（STRIPAK）复合物的核心成分，该复合物参与胚胎发生与发育、昼夜节律、2型糖尿病和癌症进展。然而，其在哺乳动物耳蜗中的表达及作用仍不清楚。在此，我们通过使用基因敲除小鼠研究了在小鼠耳蜗中的表达和功能。我们首先发现，从出生后第3天（P3）开始，随着小鼠年龄增长，的mRNA和蛋白表达增加，并在P30时达到最高表达水平，且从P14开始仅在耳蜗毛细胞（HCs）中能通过免疫荧光染色检测到的表达，而在支持细胞（SCs）中未检测到。接下来，我们将杂合基因敲除（+/-）小鼠进行杂交以获得纯合基因敲除（-/-）小鼠，用于研究在耳蜗毛细胞中的作用。然而，未获得-/-小鼠，每窝+/-小鼠与+/+小鼠的比例约为2:1，这表明纯合基因敲除具有胚胎致死性。我们测量了P30和P60的+/-小鼠的听力功能并计数了毛细胞数量，发现与+/+小鼠相比，它们具有正常的听力能力和毛细胞数量。我们的研究表明，可能在毛细胞的发育和成熟中发挥重要作用，这有待未来进一步研究。
