Striatin-interacting protein 1 () is a core component of the striatin interacting phosphatase and kinase (STRIPAK) complex, which is involved in embryogenesis and development, circadian rhythms, type 2 diabetes, and cancer progression. However, the expression and role of in the mammalian cochlea remains unclear. Here we studied the expression and function of in the mouse cochlea by using knockout mice. We first found that the mRNA and protein expression of increases as mice age starting from postnatal day (P) 3 and reaches its highest expression level at P30 and that the expression of can be detected by immunofluorescent staining starting from P14 only in cochlear HCs, and not in supporting cells (SCs). Next, we crossed heterozygous knockout ( +/-) mice to obtain homozygous knockout (-/-) mice for studying the role of in cochlear HCs. However, no -/- mice were obtained and the ratio of +/- to +/+ mice per litter was about 2:1, which suggested that homozygous knockout is embryonic lethal. We measured hearing function and counted the HC number in P30 and P60 +/- mice and found that they had normal hearing ability and HC numbers compared to +/+ mice. Our study suggested that probably play important roles in HC development and maturation, which needs further study in the future.