Reiter Sinah, Dunkel Andreas, Metwaly Amira, Panes Julian, Salas Azucena, Haller Dirk, Hofmann Thomas
Chair for Food Chemistry and Molecular Sensory Science, Technical University of Munich, Lise-Meitner-Straße 34, D-85354 Freising, Germany.
ZIEL-Institute for Food and Health, Technical University of Munich, Weihenstephaner Berg 1, 85354 Freising, Germany.
J Agric Food Chem. 2021 May 5;69(17):5238-5251. doi: 10.1021/acs.jafc.1c00769. Epub 2021 Apr 23.
In addition to their important role in fat digestion, bile acids are increasingly being used as markers for various diseases. The large diversity of bile acids results from the conversion of primary and conjugated bile acids into secondary bile acids by deconjugation and dehydroxylation reactions mediated by the intestinal microbiota. Here, we describe a fast and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for absolute quantitation of 45 bile acids in human or mouse feces in combination with a simple workup and extraction procedure. Method validation outlined excellent limits of detection and quantitation, linearity, selectivity, recovery, extraction loss, and precision. To investigate the connection between microbiome alterations and bile acid metabolism, the method was applied on a Crohn's disease study including patients with histologically documented active disease or remission as well as on a model using humanized mice. As the complex mechanism including genetic and environmental factors leading to the development of Crohn's disease is so far not completely understood, the study investigates the microbial metabolism of bile acids and the potential use of bile acid profiles to predict disease state.
除了在脂肪消化中发挥重要作用外，胆汁酸越来越多地被用作各种疾病的标志物。胆汁酸的多样性源于肠道微生物群介导的去结合和去羟基化反应，将初级和结合胆汁酸转化为次级胆汁酸。在此，我们描述了一种快速灵敏的液相色谱-串联质谱（LC-MS/MS）方法，用于绝对定量人或小鼠粪便中的45种胆汁酸，并结合简单的预处理和提取程序。方法验证显示出出色的检测限和定量限、线性、选择性、回收率、提取损失和精密度。为了研究微生物群改变与胆汁酸代谢之间的联系，该方法应用于一项克罗恩病研究，包括组织学记录为活动性疾病或缓解期的患者，以及一项使用人源化小鼠的模型。由于导致克罗恩病发生的包括遗传和环境因素在内的复杂机制目前尚未完全了解，该研究调查了胆汁酸的微生物代谢以及胆汁酸谱预测疾病状态的潜在用途。
