National Engineering Laboratory for Modern Silk, College of Textile & Clothing Engineering, Soochow University, Industrial Park, Suzhou, 215123, Jiangsu, China.
Cell & Molecular Biology Institute, College of Medicine, Soochow University, Suzhou, 215123, Jiangsu, China.
Nanomedicine (Lond). 2021 Apr;16(10):839-853. doi: 10.2217/nnm-2020-0481. Epub 2021 Apr 23.
To obtain a gene carrier that can effectively deliver loaded therapeutic genes to tumor cells, avoid toxic effects on normal cells and reduce nonspecific adsorption of plasma proteins. The conjugate of poly(ethylene glycol) (PEG) and MMP2SSP (PEG-MMP2SSP) was covalently coupled to cationized silk fibroin (CASF) through disulfide bond exchange reaction to obtain a PEG-MMP2SSP-modified CASF (CASFMP). The PEG chains were effectively cleaved from the CASFMP by MMP2. CASFMP/pDNA complexes inhibited human fibrosarcoma cell proliferation, and its cytotoxicity to human normal embryonic kidney cells was significantly lower than that of poly(ethylenimine)/pDNA after coculturing with cells for 24 h. CASFMP is a promising compound for use in gene therapy.
为了获得一种能够将负载的治疗基因有效递送至肿瘤细胞的基因载体，避免对正常细胞产生毒性作用，并减少血浆蛋白的非特异性吸附。通过二硫键交换反应将聚乙二醇（PEG）和 MMP2SSP（PEG-MMP2SSP）的缀合物共价偶联到阳离子化丝素蛋白（CASF）上，得到 PEG-MMP2SSP 修饰的 CASF（CASFMP）。MMP2 可有效地将 PEG 链从 CASFMP 上切割下来。CASFMP/pDNA 复合物抑制人纤维肉瘤细胞增殖，与细胞共培养 24 小时后，其对人正常胚胎肾细胞的细胞毒性明显低于聚（乙二胺）/pDNA。CASFMP 是一种有前途的基因治疗用化合物。
