Hjøllund E, Richelsen B, Pedersen O
Department of Internal Medicine, Aarhus Amtssygehus, Denmark.
Acta Endocrinol (Copenh). 1988 May;118(1):59-67. doi: 10.1530/acta.0.1180059.
We have described the receptor binding of A14-labelled [125I]insulin to viable adipocytes, hepatocytes, monocytes and erythrocytes from the pig. For all cell types the binding was of high affinity, specific for insulin, the non-specific binding low and degradation of insulin in the medium was minimal. At 24 degrees C, steady state insulin binding was achieved in all four cell types. At 37 degrees C, steady state insulin binding could be measured to adipocytes and hepatocytes. Specific insulin binding levels and receptor affinity for blood and fat cells from the pig are comparable to that in human cells, whereas differences, especially according to affinity, exist between pig and rat cell insulin receptor binding. It is therefore concluded that the pig is a more suitable model for studies of insulin binding in man than rodents. Finally, no correlations between the individual binding levels to the different cell types were observed. Hence, measurement of insulin binding to the easier available blood cells cannot replace studies of insulin binding to target cells of insulin.
我们已经描述了用A14标记的[125I]胰岛素与猪的活脂肪细胞、肝细胞、单核细胞和红细胞的受体结合情况。对于所有细胞类型，这种结合具有高亲和力、对胰岛素具有特异性、非特异性结合低，并且培养基中胰岛素的降解极少。在24摄氏度时，所有四种细胞类型均达到了稳态胰岛素结合。在37摄氏度时，可测量脂肪细胞和肝细胞的稳态胰岛素结合。猪的血液和脂肪细胞的特异性胰岛素结合水平和受体亲和力与人细胞中的相当，而猪和大鼠细胞胰岛素受体结合之间存在差异，尤其是在亲和力方面。因此得出结论，与啮齿动物相比，猪是研究人类胰岛素结合更合适的模型。最后，未观察到不同细胞类型的个体结合水平之间的相关性。因此，测量胰岛素与更容易获得的血细胞的结合不能替代对胰岛素与胰岛素靶细胞结合的研究。
