ARC Training Centre for Portable Analytical Separation Technologies (ASTech), Australia; Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences (Chemistry), University of Tasmania, Private Bag 75, Hobart, Tasmania 7001, Australia; Department of Analytical Chemistry, Faculty of Pharmacy, Mansoura University, 35516, Egypt.
ARC Training Centre for Portable Analytical Separation Technologies (ASTech), Australia; Trajan Scientific and Medical, Ringwood, VIC, 3134, Australia.
J Chromatogr A. 2021 Jun 7;1646:462086. doi: 10.1016/j.chroma.2021.462086. Epub 2021 Mar 22.
Stand-alone electrospray ionization mass spectrometry (ESI-MS) has been advancing through enhancements in throughput, selectivity and sensitivity of mass spectrometers. Unlike traditional MS techniques which usually require extensive offline sample preparation and chromatographic separation, many sample preparation techniques are now directly coupled with stand-alone MS to enable outstanding throughput for bioanalysis. In this review, we summarize the different sample clean-up and/or analyte enrichment strategies that can be directly coupled with ESI-MS and nano-ESI-MS for the analysis of biological fluids. The overview covers the hyphenation of different sample preparation techniques including solid phase extraction (SPE), solid phase micro-extraction (SPME), slug flow micro-extraction/nano-extraction (SFME/SFNE), liquid extraction surface analysis (LESA), extraction electrospray, extraction using digital microfluidics (DMF), and electrokinetic extraction (EkE) with ESI-MS and nano-ESI-MS.
独立式电喷雾电离质谱（ESI-MS）在提高质谱仪的通量、选择性和灵敏度方面取得了进展。与传统的 MS 技术通常需要广泛的离线样品制备和色谱分离不同，许多样品制备技术现在直接与独立式 MS 耦合，以实现生物分析的出色通量。在这篇综述中，我们总结了可直接与 ESI-MS 和纳喷电喷雾质谱联用的不同样品净化和/或分析物富集策略，用于生物体液的分析。综述涵盖了不同样品制备技术的联用，包括固相萃取（SPE）、固相微萃取（SPME）、推注流微萃取/纳流萃取（SFME/SFNE）、液体提取表面分析（LESA）、提取电喷雾、使用数字微流控（DMF）提取以及电动力学提取（EkE）与 ESI-MS 和纳喷电喷雾质谱的联用。
