School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Research Institute for Food Nutrition and Human Health, Guangzhou, China.
Bioresour Technol. 2021 Aug;333:125151. doi: 10.1016/j.biortech.2021.125151. Epub 2021 Apr 20.
To achieve ultrahigh-efficient ammonium removal and valuable biomass coproduction, Chlorella-mediated short-time acclimation was implemented in photo-fermentation. The results demonstrated short-time acclimation of mixotrophic Chlorella pyrenoidosa could significantly improve NH removal and biomass production in shake flasks. After acclimation through two batch cultures in 5-L photo-fermenter, the maximum NH removal rate (1,400 mg L d) were achieved under high NH level (4,750 mg L) in batch 3. In 50-L photo-fermenter, through one batch acclimated culture, the maximum NH removal rate (2,212 mg L d) and biomass concentration (58.4 g L) were achieved in batch 2, with the highest productivities of protein (5.56 g L d) and total lipids (5.66 g L d). The hypothetical pathway of nutrients assimilation in mixotrophic cells as cell factory was proposed with detailed discussion. This study provided a novel strategy for high-ammonium wastewater treatment without dilution, facilitating the algae-based "waste-to-treasure" bioconversion process for green manufacturing.
为实现超高效率的铵去除和有价值的生物质共生产，在光发酵中实施了小球藻介导的短时间驯化。结果表明，混养小球藻的短时间驯化可以显著提高摇瓶中的 NH 去除率和生物量生产。在 5-L 光发酵罐中经过两次分批培养驯化后，在第 3 批中在高 NH 水平（4750mg/L）下达到最大 NH 去除率（1400mg L d）。在 50-L 光发酵罐中，通过一次批驯化培养，在第 2 批中达到最大 NH 去除率（2212mg L d）和生物量浓度（58.4g/L），蛋白质（5.56g/L d）和总脂质（5.66g/L d）的最高生产力。提出了一种用于兼养细胞作为细胞工厂的养分同化假设途径，并进行了详细讨论。这项研究为高浓度氨废水处理提供了一种不稀释的新策略，促进了基于藻类的“废物变宝”生物转化过程，实现绿色制造。
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