School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China.
Bioresour Technol. 2020 Oct;313:123499. doi: 10.1016/j.biortech.2020.123499. Epub 2020 May 7.
To achieve a high consumption rate of ammonium with biomass coproduction, the mixotroph Chlorella pyrenoidosa was cultivated in high ammonium-high salinity wastewater medium in this study. The initial cell density, glucose and ammonium concentrations, and light intensity were optimized in shake flasks. A 5-L fermenter with surrounding LED (Light Emitting Diode) and a 50-L fermenter with inlet LED were employed for batch and semicontinuous cultivation. The results demonstrated that the highest contents of protein (56.7% DW) and total pigments (4.48% DW) with productivities of 5.62 g L d and 0.55 mg L d, respectively, were obtained in 5-L photofermenter, while the maximum NH consumption rate (1,800 mg L d) and biomass yield (23.6 g L) were achieved in 50-L photofermenter. This study developed a novel strategy to convert high ammonium in wastewater to high-protein algal biomass, facilitating wastewater bioremediation and nitrogen recycling utilization by the mixotroph C. pyrenoidosa in photofermentation.
为了实现生物质共生产过程中铵盐的高消耗率，本研究采用高光强条件下高浓度铵盐和高盐废水作为培养介质，培养混养绿球藻。通过摇瓶实验优化了初始细胞密度、葡萄糖和铵盐浓度以及光照强度等培养条件。采用 5-L 周边 LED 光照发酵罐和 50-L 进液 LED 光照发酵罐进行分批和半连续培养实验。结果表明，在 5-L 光发酵罐中获得了最高的蛋白含量（56.7%DW）和总类胡萝卜素含量（4.48%DW），产物得率分别为 5.62 g L d 和 0.55 mg L d。而在 50-L 光发酵罐中，最大的 NH 消耗速率（1800 mg L d）和生物量产量（23.6 g L）。本研究开发了一种将废水中高浓度铵盐转化为高蛋白藻类生物质的新策略，通过混养绿球藻在光发酵过程中实现了废水的生物修复和氮的循环利用。
