School of Veterinary Science, Faculty of Science, University of Sydney, Brownlow Hill, 2570, New South Wales, Australia,
The Unusual Pet Vets, Melbourne 3930, Victoria, Australia.
J Avian Med Surg. 2021 Apr;35(1):37-44. doi: 10.1647/1082-6742-35.1.37.
is an ascomycete yeast often found at the isthmus of the ventriculus and proventriculus of infected birds. Antemortem diagnosis has traditionally involved direct visualization of organisms on wet-mount or gram-stained fecal preparations, cloacal and crop swabs, or by both methods; however, different in-clinic diagnostic techniques have never been compared to establish an optimum test for the identification of in an avian patient. We compared 5 microscopically evaluated diagnostic testing methods: fecal Gram's stain, direct fecal wet preparation, macro suspension technique, macro suspension with Gram's stain, and macro suspension stained with new methylene blue. Each technique was performed on 96 fecal samples collected during the treatment of -infected budgerigars with water-soluble amphotericin B. The macro suspension technique produced statistically higher organism counts than the other 4 techniques and was always estimated to have the largest detection probability. We recommend that the macro suspension technique be implemented as the most efficacious diagnostic test for in-clinic assessment of avian patients possibly infected with .
是一种子囊菌酵母，通常存在于受感染鸟类的中肠和前胃的峡部。传统的生前诊断包括直接观察湿片或革兰氏染色粪便标本、泄殖腔和嗉囊拭子上的微生物；然而，从未比较过不同的临床诊断技术，以确定用于鉴定鸟类中 的最佳检测方法。我们比较了 5 种显微镜评估诊断检测方法：粪便革兰氏染色、直接粪便湿片、宏观悬浮技术、革兰氏染色宏观悬浮和新亚甲基蓝染色宏观悬浮。每种技术都在 96 份治疗感染水合两性霉素 B 的虎皮鹦鹉的粪便样本上进行。宏观悬浮技术产生的微生物计数明显高于其他 4 种技术，并且总是估计具有最大的检测概率。我们建议将宏观悬浮技术作为最有效的临床诊断检测方法，用于评估可能感染 的鸟类患者。
