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[利用草酸氧化酶的酶法测定尿液中的草酸盐:酶法与气相色谱法的比较]

[The determination of oxalate in urine by enzymatic method using oxalate oxidase: comparison between enzymatic and gas-chromatographic methods].

作者信息

Yamamoto I, Yanagawa M, Yamakawa K, Kawamura J

机构信息

Department of Urology, Mie University School of Medicine.

出版信息

Hinyokika Kiyo. 1988 Mar;34(3):419-22.

PMID:3389288
Abstract

Urinary oxalate is one of the most important constituents of urolithiasis, but the determination of oxalate in urine has not been performed as a routine laboratory examination. We tried to measure oxalate in urine by the enzymatic method with oxalate oxidase. The linearity of the standard curve and reproducibility of this method were confirmed. (Linearity: r = 0.996, S.D./mean: 0.5-3.6%, recovery rate: 99.5 +/- 3.3 (mean +/- S.D.)%) The correlation between this method and gas-chromatographic method was 0.926. The enzymatic method with oxalate oxidase can be utilized for determining the urinary oxalate as a routine laboratory examination.

摘要

尿草酸是尿路结石最重要的成分之一,但尿中草酸的测定尚未作为常规实验室检查项目开展。我们尝试用草酸氧化酶的酶法来测定尿中的草酸。该方法的标准曲线线性及重现性得到了证实。(线性:r = 0.996,标准差/均值:0.5 - 3.6%,回收率:99.5 ± 3.3(均值 ± 标准差)%)该方法与气相色谱法之间的相关性为0.926。草酸氧化酶酶法可作为常规实验室检查用于测定尿草酸。

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