结核分枝杆菌外泌体中的 Hsp16.3 作为结核病的生物标志物。
Hsp16.3 of mycobacterium tuberculosis in exosomes as a biomarker of tuberculosis.
机构信息
CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, People's Republic of China.
Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University; Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing, 101149, China.
出版信息
Eur J Clin Microbiol Infect Dis. 2021 Nov;40(11):2427-2430. doi: 10.1007/s10096-021-04246-x.
Exosomes are selectively packaged cell-derived vesicles that contain a rich cargo of nucleic acids and proteins. The small heat shock protein, Hsp16.3, is an important capsule protein produced by Mycobacterium tuberculosis (MTB). Exploring the distribution of Hsp16.3 in exosomes is valuable to tuberculosis biomarker development. Our results showed that Hsp16.3 protein overexpressed in cells can be efficiently packaged into exosomes. U937 cells infected with MTB secreted abnormally excessive amounts of Hsp16.3 protein in exosomes. Finally, a substantial number of Hsp16.3 proteins were detected in blood exosomes of tuberculosis patients. The research provides a potential exosome-based tuberculosis biomarker for MTB diagnosis.
外泌体是一种选择性包装的细胞衍生小泡，其中包含丰富的核酸和蛋白质 cargo。小热休克蛋白 Hsp16.3 是由结核分枝杆菌（MTB）产生的一种重要囊泡蛋白。探索 Hsp16.3 在 exosomes 中的分布对于开发结核病生物标志物很有价值。我们的结果表明，细胞中过表达的 Hsp16.3 蛋白可以有效地被包装到 exosomes 中。感染 MTB 的 U937 细胞分泌的 exosomes 中 Hsp16.3 蛋白异常过量。最后，在结核病患者的血液 exosomes 中检测到大量的 Hsp16.3 蛋白。该研究为 MTB 诊断提供了一种潜在的基于外泌体的结核病生物标志物。
Zotero 插件