Yang Juan, Chen Gang, Guo Ting-Wang, Qin Wen-Yi, Jia Ping
Department of Combination of Chinese and Western Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Key Laboratory of Natural Medicine Research of Chongqing Education Commission, College of Environment and Resources, Chongqing Technology and Business University, Chongqing, 400067, China.
J Ethnopharmacol. 2021 Jul 15;275:114123. doi: 10.1016/j.jep.2021.114123. Epub 2021 Apr 22.
Simiao Wan (SMW) is a classical traditional Chinese medicine (TCM) prescription to empirically treat gouty arthritis (GA) in TCM clinical practice. However, the potential mechanisms of SMW on GA are not fully evaluated.
The aim of this study is to investigate the role of macrophage polarization in the anti-GA activity of SMW.
Rats were intragastricly treated with SMW for consecutive 7 days. On day 6, monosodium urate (MSU) crystal-induced arthritis (MIA) in the ankle joint was prepared. Paw volume, gait score and histological score were measured. Levels of interleukin (IL)-1β and IL-10 in serum were detected by enzyme-linked immunosorbent assay. Expressions of inducible nitric oxide synthase (iNOS), arginase (Arg)-1, phosphorylated (p)-p65, inhibitor of nuclear factor (NF)-κB (IκB)α, p-signal transducer and transcription activator (STAT)3 and p-Janus kinase (JAK)2 in synovial tissues were determined by Western blot.
The elevated paw volume, gait score and histological score in MIA rats were significantly decreased by SMW treatment. Meanwhile, SMW significantly decreased the IL-1β level and increased the IL-10 level in serum of MIA rats. Furthermore, SMW reduced the expressions of iNOS, p-p65 and enhanced the expressions of Arg-1, IκBα, p-STAT3 and p-JAK2 in synovial tissues of MIA rats.
The results suggest that SMW attenuates the inflammation in MIA rats through promoting macrophage M2 polarization.
四妙丸（SMW）是中医临床实践中经验性治疗痛风性关节炎（GA）的经典中药方剂。然而，SMW治疗GA的潜在机制尚未得到充分评估。
本研究旨在探讨巨噬细胞极化在SMW抗GA活性中的作用。
大鼠连续7天灌胃给予SMW。在第6天，制备踝关节尿酸钠（MSU）晶体诱导的关节炎（MIA）。测量爪体积、步态评分和组织学评分。采用酶联免疫吸附测定法检测血清中白细胞介素（IL）-1β和IL-10水平。通过蛋白质免疫印迹法测定滑膜组织中诱导型一氧化氮合酶（iNOS）、精氨酸酶（Arg）-1、磷酸化（p）-p65、核因子（NF）-κB抑制剂（IκB）α、磷酸化信号转导和转录激活因子（STAT）3以及磷酸化Janus激酶（JAK）2的表达。
SMW治疗显著降低了MIA大鼠升高的爪体积、步态评分和组织学评分。同时，SMW显著降低了MIA大鼠血清中IL-1β水平并提高了IL-10水平。此外，SMW降低了MIA大鼠滑膜组织中iNOS、p-p65的表达，并增强了Arg-1、IκBα、p-STAT3和p-JAK2的表达。
结果表明，SMW通过促进巨噬细胞M2极化减轻MIA大鼠的炎症反应。
