Laboratory of Enology and Applied Microbiology, Institute of Biotechnology, University of Caxias do Sul, Brazil.
Laboratory of Enology and Applied Microbiology, Institute of Biotechnology, University of Caxias do Sul, Brazil.
Int J Food Microbiol. 2021 Jun 2;347:109200. doi: 10.1016/j.ijfoodmicro.2021.109200. Epub 2021 Apr 15.
The physicochemical characteristics and yeasts diversity in honey samples from 17 species of stingless bees of the genera Nannotrigona, Melipona, Plebeia, Scraptotrigona, and Tetragonisca cultivated in Southern Brazil were determined. The sugar content, moisture, water activity, pH, reducing sugars/total sugar ratio, and total yeast population varied significantly among the honey from the different bee species. The highest yeast population was found in the Plebeia's honey samples and correlated with their high water-activity. Sixteen yeast species were identified based on the nuclear large subunit (26S) ribosomal RNA partial sequences. The genera Starmerella and Zygosaccharomyces were found predominant, with a high prevalence of Starmerella sp., S. etchellsii, and S. apicola. Some yeast species were only identified in honey samples from specific bee species indicating a close relationship between the yeasts and the insects. For the first time, Wickerhamomyces sydowiorum in honey is being reported. In general, the yeast species isolated from stingless bee honey samples demonstrated high osmotolerance and low sugar assimilation.
测定了产自巴西南部的 17 种无刺蜜蜂属（Nannotrigona、Melipona、Plebeia、Scraptotrigona 和 Tetragonisca）的蜂蜜样本的理化特性和酵母多样性。不同蜜蜂种类的蜂蜜在糖含量、水分、水活度、pH 值、还原糖/总糖比和总酵母种群方面存在显著差异。 Plebeia 的蜂蜜样本中发现了最高的酵母种群，这与其高水活度有关。根据核大亚基（26S）核糖体 RNA 部分序列鉴定出了 16 种酵母。属 Starmerella 和 Zygosaccharomyces 占优势，其中 Starmerella sp.、S. etchellsii 和 S. apicola 较为常见。一些酵母仅在特定蜜蜂种类的蜂蜜样本中被鉴定出来，表明酵母与昆虫之间存在密切关系。首次报道了在蜂蜜中发现的 Wickerhamomyces sydowiorum。总的来说，从无刺蜜蜂蜂蜜样本中分离出的酵母种类表现出较高的耐渗透压性和低的糖同化能力。
