School of Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu, China;Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, Qinghai, China.
Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, Qinghai, China.
Biomed Environ Sci. 2021 Apr 20;34(4):299-308. doi: 10.3967/bes2021.039.
To investigate reciprocal regulation between Fur and two RyhB homologs in ( ), as well as the roles of RyhBs in biofilm formation.
Regulatory relationships were assessed by a combination of colony morphology assay, primer extension, electrophoretic mobility shift assay and DNase I footprinting.
Fur bound to the promoter-proximal DNA regions of and to repress their transcription, while both RyhB1 and RyhB2 repressed the expression of Fur at the post-transcriptional level. In addition, both RyhB1 and RyhB2 positively regulated biofilm exopolysaccharide (EPS) production and the expression of and .
Fur and the two RyhB homologs exert negative reciprocal regulation, and RyhB homologs have a positive regulatory effect on biofilm formation in .
研究（ ）中 Fur 与两个 RyhB 同源物之间的相互调节作用，以及 RyhBs 在生物膜形成中的作用。
通过菌落形态测定、引物延伸、电泳迁移率变动分析和 DNase I 足迹实验相结合的方法评估调控关系。
Fur 与 和 的启动子近端 DNA 区域结合，抑制其转录，而 RyhB1 和 RyhB2 均在转录后水平抑制 Fur 的表达。此外，RyhB1 和 RyhB2 均正向调节 生物膜胞外多糖（EPS）的产生以及 和 的表达。
Fur 和两个 RyhB 同源物之间存在负向相互调节作用，RyhB 同源物对 的生物膜形成有正向调节作用。
