• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于中国仓鼠卵巢细胞生长及重组水痘-带状疱疹病毒糖蛋白E表达的培养基优化

Culture media optimization for Chinese hamster ovary cell growth and expression of recombinant varicella-zoster virus glycoprotein E.

作者信息

Kim Kwang Sung, Park Shin Ae, Wui Seo Ri, Ko Ara, Lee Na Gyong

机构信息

Department of Integrated Bioscience & Biotechnology, Sejong University, 209 Neungdong-ro, Gwangjin-gu, Seoul, 05006 Republic of Korea.

R&D Center, EyeGene, Goyang, 10551 Republic of Korea.

出版信息

Cytotechnology. 2021 Jun;73(3):433-445. doi: 10.1007/s10616-021-00468-1. Epub 2021 Apr 20.

DOI:10.1007/s10616-021-00468-1
PMID:33897103
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8056999/
Abstract

UNLABELLED

Herpes zoster (HZ) is caused by reactivation of varicella-zoster virus (VZV) latent in the sensory ganglia and causes severe pain, often leading to postherpetic neuralgia (PHN). Two prophylactic vaccines against HZ are currently licensed for human use, a live attenuated vaccine and a subunit vaccine containing recombinant VZV glycoprotein E (gE) as antigen. The latter has superior protective efficacy against HZ and PHN. During HZ subunit vaccine development, we obtained Chinese hamster ovary (CHO) cell clones expressing VZV gE. This study was performed to optimize culture media conditions for CHO cell growth and gE production. Using a high-throughput culture system, three CHO cell clones were cultured in microtiter plates containing 24 different basal media, and three basal media were selected. The clone with the highest gE expression was fed-batch cultured in each of the three basal media in combination with 13 different feed media. A pair of media, BalanCD CHO Growth A and EX-CELL Advanced CHO Feed 1, with the highest productivity was selected for gE production. Scale-up fed-batch cultures of the selected clone cultured in a wave bag bioreactor containing the optimized media yielded 2440 mg gE protein/L culture, a 11.5-fold increase compared to original culture conditions (batch culture in CD OptiCHO medium). The optimized media condition is used to produce VZV gE antigen for an HZ subunit vaccine, which is under phase I clinical trial. This study would provide valuable insights on culture media optimization for CHO cells expressing a recombinant vaccine antigen.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s10616-021-00468-1.

摘要

未标注

带状疱疹（HZ）由潜伏在感觉神经节中的水痘-带状疱疹病毒（VZV）重新激活引起，会导致严重疼痛，常引发带状疱疹后神经痛（PHN）。目前有两种预防HZ的疫苗已获许可用于人类，一种是减毒活疫苗，另一种是含有重组VZV糖蛋白E（gE）作为抗原的亚单位疫苗。后者对HZ和PHN具有更高的保护效力。在HZ亚单位疫苗研发过程中，我们获得了表达VZV gE的中国仓鼠卵巢（CHO）细胞克隆。本研究旨在优化CHO细胞生长和gE生产的培养基条件。使用高通量培养系统，将三个CHO细胞克隆在含有24种不同基础培养基的微量滴定板中培养，筛选出三种基础培养基。将gE表达量最高的克隆在这三种基础培养基中分别与13种不同的补料培养基进行补料分批培养。选择了一对生产率最高的培养基，即BalanCD CHO Growth A和EX-CELL Advanced CHO Feed 1，用于gE生产。在装有优化培养基的波浪袋生物反应器中对所选克隆进行放大补料分批培养，产生了2440 mg gE蛋白/L培养物，与原始培养条件（在CD OptiCHO培养基中进行分批培养）相比增加了11.5倍。优化后的培养基条件用于生产处于I期临床试验阶段的HZ亚单位疫苗的VZV gE抗原。本研究将为表达重组疫苗抗原的CHO细胞的培养基优化提供有价值的见解。

补充信息

在线版本包含可在10.1007/s10616-021-00468-1获取的补充材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975a/8167074/6f5e9d35361b/10616_2021_468_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975a/8167074/44e165cf6547/10616_2021_468_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975a/8167074/dc11a2103e92/10616_2021_468_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975a/8167074/77bf0dede397/10616_2021_468_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975a/8167074/2e4b7c3b5f36/10616_2021_468_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975a/8167074/b6cd78401641/10616_2021_468_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975a/8167074/7cb0850b7e55/10616_2021_468_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975a/8167074/6f5e9d35361b/10616_2021_468_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975a/8167074/44e165cf6547/10616_2021_468_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975a/8167074/dc11a2103e92/10616_2021_468_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975a/8167074/77bf0dede397/10616_2021_468_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975a/8167074/2e4b7c3b5f36/10616_2021_468_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975a/8167074/b6cd78401641/10616_2021_468_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975a/8167074/7cb0850b7e55/10616_2021_468_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/975a/8167074/6f5e9d35361b/10616_2021_468_Fig7_HTML.jpg

相似文献

1
Culture media optimization for Chinese hamster ovary cell growth and expression of recombinant varicella-zoster virus glycoprotein E.用于中国仓鼠卵巢细胞生长及重组水痘-带状疱疹病毒糖蛋白E表达的培养基优化
Cytotechnology. 2021 Jun;73(3):433-445. doi: 10.1007/s10616-021-00468-1. Epub 2021 Apr 20.
2
Efficient induction of cell-mediated immunity to varicella-zoster virus glycoprotein E co-lyophilized with a cationic liposome-based adjuvant in mice.在小鼠中，与基于阳离子脂质体的佐剂共冻干的水痘带状疱疹病毒糖蛋白E可有效诱导细胞介导的免疫。
Vaccine. 2019 Apr 3;37(15):2131-2141. doi: 10.1016/j.vaccine.2019.02.048. Epub 2019 Feb 28.
3
Enhanced Potency and Persistence of Immunity to Varicella-Zoster Virus Glycoprotein E in Mice by Addition of a Novel BC02 Compound Adjuvant.通过添加新型BC02复合佐剂增强小鼠对水痘带状疱疹病毒糖蛋白E的免疫效力和持久性
Vaccines (Basel). 2022 Mar 29;10(4):529. doi: 10.3390/vaccines10040529.
4
Evaluation of glycoprotein E subunit and live attenuated varicella-zoster virus vaccines formulated with a single-strand RNA-based adjuvant.评价基于单链 RNA 佐剂的糖蛋白 E 亚单位和减毒活水痘带状疱疹病毒疫苗。
Immun Inflamm Dis. 2020 Jun;8(2):216-227. doi: 10.1002/iid3.297. Epub 2020 Mar 13.
5
Recombinant Glycoprotein E of Varicella Zoster Virus Contains Glycan-Peptide Motifs That Modulate B Cell Epitopes into Discrete Immunological Signatures.水痘带状疱疹病毒糖蛋白 E 的重组蛋白包含糖肽基序，可将 B 细胞表位调节为离散的免疫学特征。
Int J Mol Sci. 2019 Feb 22;20(4):954. doi: 10.3390/ijms20040954.
6
Immunogenicity in Mice Immunized with Recombinant Adenoviruses Expressing Varicella-Zoster Virus Envelope Glycoprotein E.重组腺病毒表达水痘-带状疱疹病毒包膜糖蛋白 E 免疫小鼠的免疫原性。
Viruses. 2023 Nov 22;15(12):2288. doi: 10.3390/v15122288.
7
Recombinant glycoprotein E produced in mammalian cells in large-scale as an antigen for varicella-zoster-virus serology.用哺乳动物细胞大规模生产的重组糖蛋白 E 作为水痘-带状疱疹病毒血清学的抗原。
J Virol Methods. 2011 Jul;175(1):53-9. doi: 10.1016/j.jviromet.2011.04.014. Epub 2011 Apr 22.
8
Herpes Zoster Vaccines.带状疱疹疫苗。
J Infect Dis. 2021 Sep 30;224(12 Suppl 2):S429-S442. doi: 10.1093/infdis/jiab387.
9
Immunogenicity of Varicella Zoster Virus DNA Vaccines Encoding Glycoprotein E and Immediate Early Protein 63 in Mice.水痘带状疱疹病毒糖蛋白 E 和早期蛋白 63 DNA 疫苗在小鼠中的免疫原性。
Viruses. 2022 Jun 2;14(6):1214. doi: 10.3390/v14061214.
10
Immunogenicity generated by mRNA vaccine encoding VZV gE antigen is comparable to adjuvanted subunit vaccine and better than live attenuated vaccine in nonhuman primates.mRNA 疫苗编码 VZV gE 抗原产生的免疫原性在非人类灵长类动物中与佐剂亚单位疫苗相当，优于减毒活疫苗。
Vaccine. 2020 Aug 10;38(36):5793-5802. doi: 10.1016/j.vaccine.2020.06.062. Epub 2020 Jul 20.

引用本文的文献

1
Prokaryote- and Eukaryote-Based Expression Systems: Advances in Post-Pandemic Viral Antigen Production for Vaccines.基于原核生物和真核生物的表达系统：后疫情时代疫苗用病毒抗原生产的进展。
Int J Mol Sci. 2024 Nov 7;25(22):11979. doi: 10.3390/ijms252211979.
2
Development of a perfusion process for serum-free adenovirus vector herpes zoster vaccine production.用于无血清腺病毒载体带状疱疹疫苗生产的灌注工艺开发。
AMB Express. 2022 May 14;12(1):58. doi: 10.1186/s13568-022-01398-7.