Sun Mengyu, Wu Jingyi, Liu Wentian
Department of Gastroenterology and Hepatology, Tianjin Medical University General Hospital, Tianjin, China.
Cent Eur J Immunol. 2021;46(1):10-16. doi: 10.5114/ceji.2021.105241. Epub 2021 Apr 18.
MicroRNAs (miRNAs) critically impact a wide array of eukaryotic developmental and physiologic processes through post-transcriptional gene silencing. In this study, we employed miRNA array and investigated in vitro the miRNA profile of immature dendritic cells (iDCs) derived from monocytes isolated from human venous blood. Our results showed that there were 379 miRNAs which were detectable in both monocytes and iDCs among the 856 miRNAs assayed, of which 155 miRNAs were detectable in monocytes while 224 miRNAs were detectable in iDCs. There were 103 miRNAs differentially expressed which could be relevant to the differentiation of iDCs from human monocytes. Sixty-two out of 103 miRNAs were upregulated whereas 41 miRNAs were downregulated. Of particular interest were the tremendous upregulation of miR122a and the downregulation of miR200c in iDCs. In addition, it was found that the strikingly downregulated miRNAs in iDCs also included miR-335, miR-514, miR-509, miR-31, miR-442b, miR-1, miR-199a, miR-203, miR-363 and miR-489 whereas the upregulation of miR-210, miR-155, miR-126, miR-139, miR-452, miR-19a, miR-25 and miR-181d were remarkable. Our results revealed a profile change of miRNAs when human iDCs were differentiated from monocytes as a result of in vitro stimulation with relevant cytokines.
微小RNA（miRNA）通过转录后基因沉默对广泛的真核生物发育和生理过程产生关键影响。在本研究中，我们采用miRNA芯片，对从人静脉血中分离的单核细胞来源的未成熟树突状细胞（iDC）的miRNA谱进行了体外研究。我们的结果显示，在检测的856个miRNA中，有379个在单核细胞和iDC中均可检测到，其中155个miRNA在单核细胞中可检测到，224个miRNA在iDC中可检测到。有103个差异表达的miRNA可能与人单核细胞向iDC的分化相关。103个miRNA中有62个上调，41个miRNA下调。特别值得关注的是，iDC中miR122a显著上调，miR200c下调。此外，还发现iDC中显著下调的miRNA还包括miR-335、miR-514、miR-509、miR-31、miR-442b、miR-1、miR-199a、miR-203、miR-363和miR-489，而miR-210、miR-155、miR-126、miR-139、miR-452、miR-19a、miR-25和miR-181d的上调则很明显。我们的结果揭示了在相关细胞因子的体外刺激下，人iDC从单核细胞分化时miRNA谱的变化。
