Lopes Ralf, Furlan João Pedro Rueda, Dos Santos Lucas David Rodrigues, Gallo Inara Fernanda Lage, Stehling Eliana Guedes
Department of Clinical Analyses, Toxicology and Food Science, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
Front Microbiol. 2021 Apr 9;12:659900. doi: 10.3389/fmicb.2021.659900. eCollection 2021.
The pandemic sequence type 131 (ST131) carrying plasmid-mediated colistin resistance genes has emerged worldwide causing extraintestinal infections, with lineages belonging to three major clades (A, B, and C). Clade B is the most prevalent in animals, contaminating associated meat products, and can be transmitted zoonotically. However, the gene has only been associated with C2 subclade so far. In this study, we performed a genomic investigation of an (strain S802) isolated from a kale crop in Brazil, which exhibited a multidrug-resistant (MDR) profile to clinically significant antimicrobials (i.e., polymyxin, broad-spectrum cephalosporins, aminoglycosides, and fluoroquinolones). Whole-genome sequencing analysis revealed that the S802 strain belonged to serotype O25:H4, ST131/CC131, phylogenetic group B2, and virotype D5. Furthermore, S802 carried the clade B-associated allele, genes encoding resistance to clinically important antimicrobials, metals, and biocides, and was phylogenetically related to human, avian, and swine ST131-22 strains. Additionally, IncHI2-IncQ1, IncF [F2:A-:B1], and ColE1-like plasmids were identified harboring , , and , respectively. The emergence of the ST131-22 sublineage carrying , , and in agricultural soil represents a threat to food and environmental safety. Therefore, a One Health approach to genomic surveillance studies is required to effectively detect and limit the spread of antimicrobial-resistant bacteria and their resistance genes.
携带质粒介导的黏菌素耐药基因的大流行序列类型131（ST131）已在全球出现，导致肠外感染，其谱系属于三个主要分支（A、B和C）。分支B在动物中最为普遍，会污染相关肉类产品，并可通过人畜共患病传播。然而，该基因迄今为止仅与C2亚分支相关。在本研究中，我们对从巴西羽衣甘蓝作物中分离出的一株（菌株S802）进行了基因组调查，该菌株对临床上重要的抗菌药物（即多粘菌素、广谱头孢菌素、氨基糖苷类和氟喹诺酮类）呈现多重耐药（MDR）谱。全基因组测序分析表明，S802菌株属于O25:H4血清型、ST131/CC131、系统发育群B2和病毒型D5。此外，S802携带与分支B相关的等位基因、编码对临床上重要抗菌药物、金属和杀生物剂耐药的基因，并且在系统发育上与人类、禽类和猪的ST131-22菌株相关。此外，还鉴定出IncHI2-IncQ1、IncF [F2:A-:B1]和ColE1样质粒，分别携带、和。携带、和的ST131-22亚系在农业土壤中的出现对食品和环境安全构成威胁。因此，需要采用“同一健康”方法进行基因组监测研究，以有效检测和限制耐药细菌及其耐药基因的传播。
