Cao Ruoyan, Liu Suyang, Zhang Jiayu, Ren Xianyue, Chen Xijuan, Cheng Bin, Xia Juan
Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.
Front Genet. 2021 Apr 9;12:630794. doi: 10.3389/fgene.2021.630794. eCollection 2021.
TP53INP2 plays an important role in regulating gene transcription and starvation-induced autophagy, however, its function in head and neck squamous cell carcinoma (HNSCC) remains unclear. Therefore, we assessed the expression and prognostic value of TP53INP2. In addition, RNAseq, miRNAseq, copy number variation, and mutation profiles from The Cancer Genome Atlas (TCGA) dataset were applied to evaluate the distinctive genomic patterns related to TP53INP2 expression. We found that TP53INP2 expression was lower in HNSCC compared with normal controls. Patients with higher TP53INP2 expression had longer survival time. Knockdown of TP53INP2 promoted cell viability. Functional analysis exhibited that TP53INP2 was linked to DNA replication, DNA repair, cell cycle, and multiple metabolic pathways. Moreover, TP53INP2 might affect the expression of multiple genes enhancing the transcriptional activity of nuclear hormone receptors. A competing endogenous RNA (ceRNA) network consisting of 33 lncRNAs, eight miRNAs, and 13 mRNAs was constructed based on the expression of TP53INP2. Taken together, our study highlights the potential value of TP53INP2 in predicting the survival of HNSCC and its important role in the genesis and development of HNSCC.
TP53INP2在调节基因转录和饥饿诱导的自噬中发挥重要作用，然而，其在头颈部鳞状细胞癌（HNSCC）中的功能仍不清楚。因此，我们评估了TP53INP2的表达及预后价值。此外，应用来自癌症基因组图谱（TCGA）数据集的RNA测序、miRNA测序、拷贝数变异和突变谱来评估与TP53INP2表达相关的独特基因组模式。我们发现，与正常对照相比，HNSCC中TP53INP2表达较低。TP53INP2表达较高的患者生存时间更长。敲低TP53INP2可促进细胞活力。功能分析表明，TP53INP2与DNA复制、DNA修复、细胞周期和多种代谢途径有关。此外，TP53INP2可能影响多个基因的表达，增强核激素受体的转录活性。基于TP53INP2的表达构建了一个由33个长链非编码RNA、8个miRNA和13个mRNA组成的竞争性内源RNA（ceRNA）网络。综上所述，我们的研究突出了TP53INP2在预测HNSCC生存方面的潜在价值及其在HNSCC发生发展中的重要作用。
