人多能干细胞在 OP9-MS5 共培养体系下向 B 细胞系的分化。
differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture.
机构信息
Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB2 0AW, UK.
Department of Haematology, University of Cambridge, Jeffrey Cheah Biomedical Centre, Cambridge CB2 0AW, UK.
出版信息
STAR Protoc. 2021 Mar 31;2(2):100420. doi: 10.1016/j.xpro.2021.100420. eCollection 2021 Jun 18.
differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize potential target cells for the initiation of childhood B acute lymphoblastic leukemia. Herein, we detail critical aspects of the protocol including reagent validation, controls, and examples of surface markers used for analysis and cell sorting. For complete details on the use and execution of this protocol, please refer to Boiers et al. (2018).
人类多能干细胞（hPSCs）的分化为研究人类组织发育和分化的生理学和病理学提供了一个遗传上可处理的系统。我们已经使用这种方法来模拟人类 B 谱系发育的最早阶段，并描述儿童 B 急性淋巴细胞白血病起始的潜在靶细胞。在此，我们详细介绍了该方案的关键方面，包括试剂验证、对照以及用于分析和细胞分选的表面标志物的示例。有关该方案使用和执行的完整详细信息，请参见 Boiers 等人（2018 年）。
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