处理人尿液和输尿管支架进行 16S rRNA 扩增子测序。
Processing human urine and ureteral stents for 16S rRNA amplicon sequencing.
机构信息
Centre for Human Microbiome and Probiotic Research, Lawson Health Research Institute, London, ON, Canada.
Department of Microbiology and Immunology, The University of Western Ontario, London, ON, Canada.
出版信息
STAR Protoc. 2021 Apr 8;2(2):100435. doi: 10.1016/j.xpro.2021.100435. eCollection 2021 Jun 18.
Ureteral stents are commonly used medical devices that harbor a unique and patient-specific microbial community. This protocol describes an optimized procedure for high-quality DNA extraction from both urine and ureteral stent samples for the purpose of downstream microbiota characterization by amplicon sequencing. Detailed instruction is provided for 16S rRNA gene V4 region sequencing with the Illumina platform, which enables accurate and reproducible microbiota profiling of low bacterial abundance urine and stent samples. For complete details on the use and execution of this protocol, please refer to Al et al. (2020).
输尿管支架是常用的医疗器械，它们携带有独特的、个体特有的微生物群落。本方案描述了一种从尿液和输尿管支架样本中提取高质量 DNA 的优化程序，目的是通过扩增子测序对下游微生物群落进行特征分析。提供了详细的 Illumina 平台 16S rRNA 基因 V4 区测序的操作说明，这使得对低细菌丰度的尿液和支架样本的微生物组进行准确和可重复的分析成为可能。有关该方案使用和实施的完整详细信息，请参考 Al 等人（2020 年）。
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