Observatoire Océanologique de Banyuls-sur-Mer, UMR CNRS 7232 BIOM, Sorbonne Université Paris, Banyuls-sur-Mer, France.
EPHE-UPVD-CNRS, USR 3278 CRIOBE, PSL University, Moorea, French Polynesia.
Dev Dyn. 2021 Nov;250(11):1651-1667. doi: 10.1002/dvdy.354. Epub 2021 May 7.
Amphiprion ocellaris is one of the rare reef fish species that can be reared in aquaria. It is increasingly used as a model species for Eco-Evo-Devo. Therefore, it is important to have an embryonic development table based on high quality images that will allow for standardized sampling by the scientific community.
Here we provide high-resolution time-lapse videos to accompany a detailed description of embryonic development in A ocellaris. We describe a series of developmental stages and we define six broad periods of embryogenesis: zygote, cleavage, blastula, gastrula, segmentation, and organogenesis that we further subdivide into 32 stages. These periods highlight the changing spectrum of major developmental processes that occur during embryonic development.
We provide an easy system for the determination of embryonic stages, enabling the development of A ocellaris as a coral reef fish model species. This work will facilitate evolutionary development studies, in particular studies of the relationship between climate change and developmental trajectories in the context of coral reefs. Thanks to its lifestyle, complex behavior, and ecology, A ocellaris will undoubtedly become a very attractive model in a wide range of biological fields.
眼斑双锯鱼是一种罕见的可在水族馆中饲养的珊瑚鱼。它越来越多地被用作生态进化发育的模式生物。因此，建立一个基于高质量图像的胚胎发育表对于科学界的标准化采样非常重要。
本文提供了高分辨率的延时视频，以及眼斑双锯鱼胚胎发育的详细描述。我们描述了一系列发育阶段，并定义了胚胎发生的六个主要时期：合子、卵裂、囊胚、原肠胚、分节和器官发生，我们进一步将其细分为 32 个阶段。这些时期突出了胚胎发育过程中发生的主要发育过程的变化范围。
我们提供了一个确定胚胎阶段的简单系统，使眼斑双锯鱼能够成为珊瑚鱼模式物种。这项工作将促进进化发育研究，特别是在珊瑚礁背景下研究气候变化与发育轨迹之间的关系。由于其生活方式、复杂行为和生态，眼斑双锯鱼无疑将成为广泛的生物学领域非常有吸引力的模型。
