Zhu Juan-Juan, Ma Hai-Jun, Zhang Xiu, Li Min, Xu Xiao-Yun, Qin Hong-Yun
College of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China.
Ningxia Grape & Wine Innovation Center, Yinchuan 750021, China.
Ying Yong Sheng Tai Xue Bao. 2021 Apr;32(4):1289-1297. doi: 10.13287/j.1001-9332.202104.021.
We investigated the effects of potassium-releasing bacteria on physiological and bioche-mical characteristics of (Cultivar Ningqi 1) under salt stress, with an experiment with treatments following randomized block design. The treatments included control (CK), 100 mmol·L NaCl stress (NaCl), 100 mmol·LNaCl stress+KSBGY01 bacteria (NaCl-M1), 100 mmol·LNaCl stress+KSBGY02 bacteria (NaCl-M2), and 100 mmol·LNaCl stress+KSBGY01+KSBGY02 (NaCl-M3). We measued chlorophyll content, polyphenol content, superoxide anion (O) content, hydrogen peroxide (HO) content, soluble sugar content, antioxidant enzyme activity and sucrose metabolic enzyme activity of seedlings. Results showed that the presence of potassium bacteria increased the values of flavonoids (FLAV), fluorescence excitation than anthocyanins relative index (FERARI), anthocyanins (ANTH-RB), nitrogen balance index (NBI-G), decreased the contents of O and HO, and improved soluble sugar content, catalase (CAT) activity, sucrose phosphate synthase (SPS) activity, sucrose synthase (SS) activity and invertase (INV) activity of leaves in seedlings under salt stress. Among all the treatments, the highest values of ANTH-RB and NBI-G, soluble sugar content, and activities of CAT, SPS, SS, and INV presented in NaCl-M2 treatment, the highest values of SPAD, FLAV, and FERARI presented in NaCl-M3 treatment, the highest activity of superoxide dismutase (SOD) presented in NaCl-M1 treatment, the highest activity of glutathione peroxidase (GSH-Px) presented in NaCl treatment, and the highest peroxisome (POD) activity presented in CK. The 14 significant physiological and biochemical indicators in the leaves of seedling were analyzed by grey system correlation degree method. Our results suggested that the weighted correlation degree of phy-siological and biochemical indices of inoculated potassium-solubilizing bacteria was higher than that under CK and NaCl treatments. The highest weighted correlation was observed in NaCl-M2 treatment. Therefore, adding KSBGY02 potassium-solubilizing bacteria could alleviate the salt stress for seedlings.
我们采用随机区组设计进行试验,研究了解钾细菌对盐胁迫下(品种宁杞1号)生理生化特性的影响。处理包括对照(CK)、100 mmol·L NaCl胁迫(NaCl)、100 mmol·L NaCl胁迫+KSBGY01细菌(NaCl-M1)、100 mmol·L NaCl胁迫+KSBGY02细菌(NaCl-M2)以及100 mmol·L NaCl胁迫+KSBGY01+KSBGY02(NaCl-M3)。我们测定了枸杞幼苗的叶绿素含量、多酚含量、超氧阴离子(O)含量、过氧化氢(H₂O₂)含量、可溶性糖含量、抗氧化酶活性和蔗糖代谢酶活性。结果表明,解钾细菌的存在增加了类黄酮(FLAV)、荧光激发与花青素相对指数(FERARI)、花青素(ANTH-RB)、氮平衡指数(NBI-G)的值,降低了O和H₂O₂的含量,并提高了盐胁迫下枸杞幼苗叶片的可溶性糖含量、过氧化氢酶(CAT)活性、蔗糖磷酸合成酶(SPS)活性、蔗糖合成酶(SS)活性和转化酶(INV)活性。在所有处理中,ANTH-RB和NBI-G、可溶性糖含量以及CAT、SPS、SS和INV活性的最高值出现在NaCl-M2处理中,SPAD、FLAV和FERARI的最高值出现在NaCl-M3处理中,超氧化物歧化酶(SOD)的最高活性出现在NaCl-M1处理中,谷胱甘肽过氧化物酶(GSH-Px)的最高活性出现在NaCl处理中,过氧化物酶(POD)的最高活性出现在CK中。采用灰色系统关联度法对枸杞幼苗叶片中的14个重要生理生化指标进行了分析。我们的结果表明,接种解钾细菌后枸杞生理生化指标的加权关联度高于CK和NaCl处理。在NaCl-M2处理中观察到最高加权关联度。因此,添加KSBGY02解钾细菌可以缓解枸杞幼苗的盐胁迫。