Department of Orthopaedic Surgery, University of Missouri, Columbia, MO, USA.
Thompson Laboratory for Regenerative Orthopaedics, University of Missouri, Columbia, MO, USA.
Cartilage. 2021 Dec;13(2_suppl):1646S-1653S. doi: 10.1177/19476035211011521. Epub 2021 Apr 26.
To evaluate differences in pro-inflammatory and degradative mediator production from osteoarthritic knee articular cartilage explants treated with a hyperosmolar saline solution supplemented with anti-inflammatory components (l-glutamine, ascorbic acid, sodium pyruvate, epigallocatechin gallate [EGCG], and dexamethasone) or normal saline using an model for knee arthroscopy.
Full-thickness 6 mm articular cartilage explants ( = 12/patient) were created from femoral condyle and tibial plateau samples collected from patients who received knee arthroplasty. One explant half was treated for 3 hours with hyperosmolar saline (600 mOsm/L) supplemented with anti-inflammatory components and the corresponding half with normal saline (308 mOsm/L). Explants were cultured for 3 days and then collected for biomarker analyses. Media biomarker concentrations were normalized to the wet weight of the tissue (mg) and were analyzed by a paired test with significance set at < 0.05.
Cartilage was collected from 9 females and 2 males (mean age = 68 years). Concentrations of MCP-1 ( < 0.001), IL-8 ( = 0.03), GRO-α ( = 0.02), MMP-1 ( < 0.001), MMP-2 ( < 0.001), and MMP-3 ( < 0.001) were significantly lower in explant halves treated with the enhanced hyperosmolar solution. When considering only those cartilage explants in the top tercile of tissue metabolism, IL-6 ( = 0.005), IL-8 ( = 0.0001), MCP-1 ( < 0.001), GRO-α ( = 0.0003), MMP-1 ( < 0.001), MMP-2 ( < 0.001), MMP-3 ( < 0.001), and GAG expression ( = 0.0001) was significantly lower in cartilage explant halves treated with the enhanced hyperosmolar solution.
Treatment of cartilage explants with a hyperosmolar saline arthroscopic irrigation solution supplemented with anti-inflammatory components was associated with significant decreases in inflammatory and degradative mediator production and mitigation of proteoglycan loss.
使用补充有抗炎成分（l-谷氨酰胺、抗坏血酸、丙酮酸钠、表没食子儿茶素没食子酸酯[EGCG]和地塞米松）的高渗盐水溶液或生理盐水溶液处理骨关节炎膝关节关节软骨的离体样本，评估其在促炎和降解介质产生方面的差异，这是一种膝关节镜术的模型。
从接受膝关节置换术的患者的股骨髁和胫骨平台样本中创建 6 毫米全厚关节软骨离体样本（每个患者 = 12 个）。将一半的离体样本用高渗盐水（600 mOsm/L）处理 3 小时，并用抗炎成分补充，另一半用生理盐水（308 mOsm/L）处理。将离体样本培养 3 天，然后收集用于生物标志物分析。将介质生物标志物浓度与组织的湿重（mg）进行归一化，并使用配对 t 检验进行分析，显著性水平设为<0.05。
从 9 名女性和 2 名男性（平均年龄=68 岁）中收集了软骨。用增强的高渗溶液处理的离体样本中，MCP-1（<0.001）、IL-8（=0.03）、GRO-α（=0.02）、MMP-1（<0.001）、MMP-2（<0.001）和 MMP-3（<0.001）的浓度明显较低。当仅考虑组织代谢最高三分位的软骨离体样本时，IL-6（=0.005）、IL-8（=0.0001）、MCP-1（<0.001）、GRO-α（=0.0003）、MMP-1（<0.001）、MMP-2（<0.001）、MMP-3（<0.001）和 GAG 表达（=0.0001）在用增强的高渗溶液处理的离体样本中明显较低。
用补充有抗炎成分的高渗盐水关节镜冲洗液处理软骨离体样本与炎症和降解介质产生的显著减少以及糖胺聚糖损失的减轻有关。
