Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA.
Department of Agronomy, Iowa State University, Ames, IA, USA.
Methods Mol Biol. 2021;2250:131-139. doi: 10.1007/978-1-0716-1134-0_13.
This chapter details the techniques used to detect transposon-induced genome rearrangements. Here, we describe a rapid DNA isolation technique, PCR amplification, and a novel High Efficiency Agarose Gel Electrophoresis Method (HEA-GEM).
本章详细介绍了用于检测转座子诱导的基因组重排的技术。在这里,我们描述了一种快速的 DNA 分离技术、PCR 扩增和一种新颖的高效琼脂糖凝胶电泳方法(HEA-GEM)。